8ITH

Crystal structure of lasso peptide epimerase MslH H295N

8ITH の概要

• エントリーDOI: 10.2210/pdb8ith/pdb
• 分子名称: Poly-gamma-glutamate synthesis protein (Capsule biosynthesis protein), GLYCEROL, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: epimerase, mslh, msla, lasso peptide, ripps, ms-271, isomerase
• 由来する生物種: Streptomyces griseorubiginosus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 47611.72

構造登録者

Nakashima, Y.,Hiroyuki, M. (登録日: 2023-03-22, 公開日: 2023-06-21, 最終更新日: 2023-08-23)

主引用文献

Nakashima, Y.,Kawakami, A.,Ogasawara, Y.,Maeki, M.,Tokeshi, M.,Dairi, T.,Morita, H.
Structure of lasso peptide epimerase MslH reveals metal-dependent acid/base catalytic mechanism.
Nat Commun, 14:4752-4752, 2023

PubMed Abstract: The lasso peptide MS-271 is a ribosomally synthesized and post-translationally modified peptide (RiPP) consisting of 21 amino acids with D-tryptophan at the C-terminus, and is derived from the precursor peptide MslA. MslH, encoded in the MS-271 biosynthetic gene cluster (msl), catalyzes the epimerization at the Cα center of the MslA C-terminal Trp21, leading to epi-MslA. The detailed catalytic process, including the catalytic site and cofactors, has remained enigmatic. Herein, based on X-ray crystallographic studies in association with MslA core peptide analogues, we show that MslH is a metallo-dependent peptide epimerase with a calcineurin-like fold. The crystal structure analysis, followed by site-directed mutagenesis, docking simulation, and ICP-MS studies demonstrate that MslH employs acid/base chemistry to facilitate the reversible epimerization of the C-terminal Trp21 of MslA, by utilizing two pairs of His/Asp catalytic residues that are electrostatically tethered to a six-coordination motif with a Ca(II) ion via water molecules.

PubMed: 37550286
DOI: 10.1038/s41467-023-40232-x

実験手法

X-RAY DIFFRACTION (2.55 Å)