8ISC

Crystal structure of MV in complex with LLP

8ISC の概要

• エントリーDOI: 10.2210/pdb8isc/pdb
• 分子名称: Branched chain amino acid: 2-keto-4-methylthiobutyrate aminotransferase (2 entities in total)
• 機能のキーワード: complex, transferase
• 由来する生物種: Mycolicibacterium vanbaalenii (strain DSM 7251 / JCM 13017 / BCRC 16820 / KCTC 9966 / NRRL B-24157 / PYR-1) (Mycobacterium vanbaalenii)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 147529.41

構造登録者

Li, Q.,Zhu, Y.M.,Gao, J.,Wei, H.L.,Han, X.,Liu, W.D.,Sun, Y.X. (登録日: 2023-03-20, 公開日: 2024-01-24, 最終更新日: 2025-08-06)

主引用文献

Zhu, Y.,Chen, P.,Dong, Q.,Li, Q.,Liu, D.,Liu, T.,Liu, W.,Sun, Y.
Protein engineering of transaminase facilitating enzyme cascade reaction for the biosynthesis of azasugars.
Iscience, 27:109034-109034, 2024

PubMed Abstract: Azasugars, such as 1-deoxynojirimycin (1-DNJ), exhibit unique physiological functions and hold promising applications in medicine and health fields. However, the biosynthesis of 1-DNJ is hindered by the low activity and thermostability of the transaminase. In this study, the transaminase from (MvTA) with activity toward d-fructose was engineered through semi-rational design and high-throughput screening method. The final mutant M9-1 demonstrated a remarkable 31.2-fold increase in specific activity and an impressive 200-fold improvement in thermostability compared to the wild-type enzyme. Molecular dynamics (MD) simulations revealed that the mutation sites of H69R and K145R in M9-1 played crucial roles in the binding of the amino acceptor and donor, leading to the stable conformation of substrates within the active pocket. An enzyme cascade reaction was developed using M9-1 and the dehydrogenase from (GutB1) for the production of mannojirimycin (MJ), which provided a new idea for the biosynthesis of 1-DNJ.

PubMed: 38433920
DOI: 10.1016/j.isci.2024.109034

実験手法

X-RAY DIFFRACTION (2.27 Å)