8IRW

Structure of nanobody Nb9 against parathion

8IRW の概要

• エントリーDOI: 10.2210/pdb8irw/pdb
• 分子名称: nanobody Nb9 against parathion (2 entities in total)
• 機能のキーワード: nanobody, parathion, immune system
• 由来する生物種: Camelus bactrianus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 36761.04

構造登録者

Wang, H.,Li, J.D.,Shen, X.,Xu, Z.L.,Sun, Y.M. (登録日: 2023-03-19, 公開日: 2024-03-20, 最終更新日: 2025-04-02)

主引用文献

Li, J.D.,Shen, X.,Xu, Z.L.,Liang, Y.F.,Shen, Y.D.,Yang, J.Y.,Wang, H.
Molecular Evolution of Antiparathion Nanobody with Enhanced Sensitivity and Specificity Based on Structural Analysis.
J.Agric.Food Chem., 71:14758-14768, 2023

PubMed Abstract: Nanobody (Nb) has gained significant attention in immunoassays owing to its numerous advantages, particularly its ease of molecular evolution. However, the limited understanding of how high sensitivity and specificity attained for antihapten Nbs hamper the development of high-performance Nbs. Herein, the antiparathion Nb (Nb9) we prepared previously was chosen as the model, and an approach based on X-ray crystallography, molecular docking, and rational site-directed saturation mutation for constructing a rapid and effective platform for nanobody evolution was described. Based on the structural analysis, two mutants, namely Nb-D5 (IC = 2.4 ± 0.2 ng/mL) and Nb-D12 (IC = 2.7 ± 0.1 ng/mL), were selected out from a six-sites directed saturation mutation library, 3.5-fold and 3.1-fold sensitivity enhancement over Nb9 to parathion, respectively. Besides, Nb-D12 exhibited improved sensitivity for quinalphos, triazophos, and coumaphos (5.4-35.4 ng/mL), indicating its broader detection potential. Overall, our study advances an effective strategy for the future rational evolution of Nbs with desirable performance.

PubMed: 37768036
DOI: 10.1021/acs.jafc.3c05176

実験手法

X-RAY DIFFRACTION (1.55 Å)