8IRP

kinetin bound state of Arabidopsis AZG1

8IRP の概要

• エントリーDOI: 10.2210/pdb8irp/pdb
• EMDBエントリー: 35682
• 分子名称: Adenine/guanine permease AZG1, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, N-(FURAN-2-YLMETHYL)-7H-PURIN-6-AMINE, ... (4 entities in total)
• 機能のキーワード: cytokinin, transporter, transport protein
• 由来する生物種: Arabidopsis thaliana (thale cress)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 132652.63

構造登録者

Xu, L.,Guo, J. (登録日: 2023-03-19, 公開日: 2024-01-17, 最終更新日: 2025-07-02)

主引用文献

Xu, L.,Jia, W.,Tao, X.,Ye, F.,Zhang, Y.,Ding, Z.J.,Zheng, S.J.,Qiao, S.,Su, N.,Zhang, Y.,Wu, S.,Guo, J.
Structures and mechanisms of the Arabidopsis cytokinin transporter AZG1.
Nat.Plants, 10:180-191, 2024

PubMed Abstract: Cytokinins are essential for plant growth and development, and their tissue distributions are regulated by transmembrane transport. Recent studies have revealed that members of the 'Aza-Guanine Resistant' (AZG) protein family from Arabidopsis thaliana can mediate cytokinin uptake in roots. Here we present 2.7 to 3.3 Å cryo-electron microscopy structures of Arabidopsis AZG1 in the apo state and in complex with its substrates trans-zeatin (tZ), 6-benzyleaminopurine (6-BAP) or kinetin. AZG1 forms a homodimer and each subunit shares a similar topology and domain arrangement with the proteins of the nucleobase/ascorbate transporter (NAT) family. These structures, along with functional analyses, reveal the molecular basis for cytokinin recognition. Comparison of the AZG1 structures determined in inward-facing conformations and predicted by AlphaFold2 in the occluded conformation allowed us to propose that AZG1 may carry cytokinins across the membrane through an elevator mechanism.

PubMed: 38172575
DOI: 10.1038/s41477-023-01590-y

実験手法

ELECTRON MICROSCOPY (2.8 Å)