8IQG

Cryo-EM structure of the monomeric human CAF1-H3-H4 complex

8IQG の概要

• エントリーDOI: 10.2210/pdb8iqg/pdb
• 関連するPDBエントリー: 7Y5K
• EMDBエントリー: 35661
• 分子名称: Chromatin assembly factor 1 subunit A, Histone H3.1, Histone H4, ... (5 entities in total)
• 機能のキーワード: histone chaperone, chromatin assembly factor, replication
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 243204.79

構造登録者

Liu, C.P.,Yu, Z.Y.,Xu, R.M. (登録日: 2023-03-16, 公開日: 2023-08-16, 最終更新日: 2023-09-06)

主引用文献

Liu, C.P.,Yu, Z.,Xiong, J.,Hu, J.,Song, A.,Ding, D.,Yu, C.,Yang, N.,Wang, M.,Yu, J.,Hou, P.,Zeng, K.,Li, Z.,Zhang, Z.,Zhang, X.,Li, W.,Zhang, Z.,Zhu, B.,Li, G.,Xu, R.M.
Structural insights into histone binding and nucleosome assembly by chromatin assembly factor-1.
Science, 381:eadd8673-eadd8673, 2023

PubMed Abstract: Chromatin inheritance entails de novo nucleosome assembly after DNA replication by chromatin assembly factor-1 (CAF-1). Yet direct knowledge about CAF-1's histone binding mode and nucleosome assembly process is lacking. In this work, we report the crystal structure of human CAF-1 in the absence of histones and the cryo-electron microscopy structure of CAF-1 in complex with histones H3 and H4. One histone H3-H4 heterodimer is bound by one CAF-1 complex mainly through the p60 subunit and the acidic domain of the p150 subunit. We also observed a dimeric CAF-1-H3-H4 supercomplex in which two H3-H4 heterodimers are poised for tetramer assembly and discovered that CAF-1 facilitates right-handed DNA wrapping of H3-H4 tetramers. These findings signify the involvement of DNA in H3-H4 tetramer formation and suggest a right-handed nucleosome precursor in chromatin replication.

PubMed: 37616371
DOI: 10.1126/science.add8673

実験手法

ELECTRON MICROSCOPY (3.5 Å)