8IQA

Crystal structure of Pyruvic Oxime Dioxygenase (POD) from Alcaligenes faecalis deleted N-terminal 18 residues

8IQA の概要

• エントリーDOI: 10.2210/pdb8iqa/pdb
• 関連するPDBエントリー: 8IL8
• 分子名称: Aldolase, NICKEL (II) ION (3 entities in total)
• 機能のキーワード: class ii aldolase-like, dioxygenase, non-heme iron, his-triad, oxidoreductase
• 由来する生物種: Alcaligenes faecalis subsp. faecalis NBRC 13111
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 108036.76

構造登録者

Tsujino, S.,Yamada, Y.,Fujiwara, T. (登録日: 2023-03-16, 公開日: 2024-03-20, 最終更新日: 2025-03-05)

主引用文献

Tsujino, S.,Yamada, Y.,Senda, M.,Nakamura, A.,Senda, T.,Fujiwara, T.
Structural characterization of pyruvic oxime dioxygenase, a key enzyme in heterotrophic nitrification.
J.Bacteriol., 207:e0034224-e0034224, 2025

PubMed Abstract: Nitrification by heterotrophic microorganisms is an important part of the nitrogen cycle in the environment. The enzyme responsible for the core function of heterotrophic nitrification is pyruvic oxime dioxygenase (POD). POD is a non-heme, Fe(II)-dependent enzyme that catalyzes the dioxygenation of pyruvic oxime to produce pyruvate and nitrite. To analyze the catalytic mechanism of POD, the crystal structure of POD from (AfPOD) was determined at 1.76 Å resolution. The enzyme is a homotetramer, and the subunit structure is homologous to those of class II aldolases, in particular, a zinc-dependent L-fuculose-1-phosphate aldolase. The active site of the subunit is located at the bottom of a cleft formed with an adjacent subunit. The iron ion at the active site is coordinated by three histidines and three water molecules in an octahedral geometry. The putative oxygen tunnel was connected between the active site and the central cavity of the tetramer. The N-terminal region of AfPOD, which is essential for catalytic activity, is disordered in the crystal. Structure prediction with AlphaFold2 combined with mutational experiments suggested that the disordered N-terminal region adopts an α-helix conformation and participates in the formation of the active site. The catalytic mechanism of the dioxygenase reaction by POD is discussed on the basis of the molecular docking model.IMPORTANCEOur knowledge of nitrification has increased considerably in recent decades with the discovery of new nitrifying microorganisms and the characterization of their biochemical processes. Some heterotrophic bacteria and fungi are known to show nitrification activities, but the molecular mechanisms have been poorly understood. Here, we performed a structural characterization of pyruvic oxime dioxygenase (POD), a key enzyme in heterotrophic nitrification that produces nitrite from ammonia using pyruvic oxime as an intermediate. Structural and enzymatic analyses revealed that POD is a unique dioxygenase with features such as an aldolase backbone, an N-terminal α-helix, and an oxygen tunnel. Our results provide insights not only into the molecular mechanisms but also into the design of specific inhibitors of heterotrophic nitrification.

PubMed: 39772954
DOI: 10.1128/jb.00342-24

実験手法

X-RAY DIFFRACTION (1.551 Å)