8IPF

Crystal structure of an ankyrin protein that can specifically recognize a parallel G-quadruplex

8IPF の概要

• エントリーDOI: 10.2210/pdb8ipf/pdb
• 関連するPDBエントリー: 8IPP
• 分子名称: DARPin (2E4) (2 entities in total)
• 機能のキーワード: g-quadruplex, parallel, dna, dna binding protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 118917.54

構造登録者

Ngo, K.H.,Liew, C.W.,Heddi, B.,Phan, A.T. (登録日: 2023-03-14, 公開日: 2024-07-31)

主引用文献

Ngo, K.H.,Liew, C.W.,Heddi, B.,Phan, A.T.
Structural Basis for Parallel G-Quadruplex Recognition by an Ankyrin Protein.
J.Am.Chem.Soc., 146:13709-13713, 2024

PubMed Abstract: G-Quadruplex (G4) structures formed by guanine-rich DNA and RNA sequences are implicated in various biological processes. Understanding the mechanisms by which proteins recognize G4 structures is crucial for elucidating their functional roles. Here we present the X-ray crystal structure of an ankyrin protein bound to a parallel G4 structure. Our findings reveal a new specific recognition mode in which a bundle of α-helices and loops of the ankyrin form a flat surface to stack on the G-tetrad core. The protein employs a combination of hydrogen bonds and hydrophobic contacts to interact with the G4, and electrostatic interaction is used to enhance the binding affinity. This binding mechanism provides valuable insights into understanding G4 recognition by proteins.

PubMed: 38738955
DOI: 10.1021/jacs.4c01971

実験手法

X-RAY DIFFRACTION (2.01 Å)