8IPA

Wheat 80S ribosome stalled on AUG-Stop boron dependently with cycloheximide

これはPDB形式変換不可エントリーです。

8IPA の概要

• エントリーDOI: 10.2210/pdb8ipa/pdb
• EMDBエントリー: 35637
• 分子名称: 18S ribosomal RNA, 40S ribosomal protein uS9, 40S ribosomal protein uS10, ... (83 entities in total)
• 機能のキーワード: ribosome, translation
• 由来する生物種: Triticum aestivum (bread wheat); 詳細
• タンパク質・核酸の鎖数: 80
• 化学式量合計: 3243448.16

構造登録者

Yokoyama, T.,Tanaka, M.,Saito, H.,Nishimoto, M.,Tsuda, K.,Sotta, N.,Shigematsu, H.,Shirouzu, M.,Iwasaki, S.,Ito, T.,Fujiwara, T. (登録日: 2023-03-14, 公開日: 2024-02-21, 最終更新日: 2024-05-15)

主引用文献

Tanaka, M.,Yokoyama, T.,Saito, H.,Nishimoto, M.,Tsuda, K.,Sotta, N.,Shigematsu, H.,Shirouzu, M.,Iwasaki, S.,Ito, T.,Fujiwara, T.
Boric acid intercepts 80S ribosome migration from AUG-stop by stabilizing eRF1.
Nat.Chem.Biol., 20:605-614, 2024

PubMed Abstract: In response to environmental changes, cells flexibly and rapidly alter gene expression through translational controls. In plants, the translation of NIP5;1, a boric acid diffusion facilitator, is downregulated in response to an excess amount of boric acid in the environment through upstream open reading frames (uORFs) that consist of only AUG and stop codons. However, the molecular details of how this minimum uORF controls translation of the downstream main ORF in a boric acid-dependent manner have remained unclear. Here, by combining ribosome profiling, translation complex profile sequencing, structural analysis with cryo-electron microscopy and biochemical assays, we show that the 80S ribosome assembled at AUG-stop migrates into the subsequent RNA segment, followed by downstream translation initiation, and that boric acid impedes this process by the stable confinement of eukaryotic release factor 1 on the 80S ribosome on AUG-stop. Our results provide molecular insight into translation regulation by a minimum and environment-responsive uORF.

PubMed: 38267667
DOI: 10.1038/s41589-023-01513-0

実験手法

ELECTRON MICROSCOPY (3.4 Å)