8IOO

Crystal structure of Deinococcus radiodurans RecJ-like protein in complex with Mg2+

8IOO の概要

• エントリーDOI: 10.2210/pdb8ioo/pdb
• 分子名称: RecJ-like protein, MAGNESIUM ION, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: rnase, nuclease, hydrolase
• 由来する生物種: Deinococcus radiodurans
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 107879.75

構造登録者

Cheng, K. (登録日: 2023-03-13, 公開日: 2024-03-20, 最終更新日: 2024-07-17)

主引用文献

Wang, Y.,Hao, W.,Guo, Z.,Sun, Y.,Wu, Y.,Sun, Y.,Gao, T.,Luo, Y.,Jin, L.,Yang, J.,Cheng, K.
Structural and functional investigation of the DHH/DHHA1 family proteins in Deinococcus radiodurans.
Nucleic Acids Res., 52:7142-7157, 2024

PubMed Abstract: DHH/DHHA1 family proteins have been proposed to play critical roles in bacterial resistance to environmental stresses. Members of the most radioresistant bacteria genus, Deinococcus, possess two DHH/DHHA1 family proteins, RecJ and RecJ-like. While the functions of Deinococcus radiodurans RecJ (DrRecJ) in DNA damage resistance have been well characterized, the role and biochemical activities of D. radiodurans RecJ-like (DrRecJ-like) remain unclear. Phenotypic and transcriptomic analyses suggest that, beyond DNA repair, DrRecJ is implicated in cell growth and division. Additionally, DrRecJ-like not only affects stress response, cell growth, and division but also correlates with the folding/stability of intracellular proteins, as well as the formation and stability of cell membranes/walls. DrRecJ-like exhibits a preferred catalytic activity towards short single-stranded RNA/DNA oligos and c-di-AMP. In contrast, DrRecJ shows no activity against RNA and c-di-AMP. Moreover, a crystal structure of DrRecJ-like, with Mg2+ bound in an open conformation at a resolution of 1.97 Å, has been resolved. Subsequent mutational analysis was conducted to pinpoint the crucial residues essential for metal cation and substrate binding, along with the dimerization state, necessary for DrRecJ-like's function. This finding could potentially extend to all NrnA-like proteins, considering their conserved amino acid sequence and comparable dimerization forms.

PubMed: 38804263
DOI: 10.1093/nar/gkae451

実験手法

X-RAY DIFFRACTION (2 Å)