8INI

vaccinia H2 protein without the transmembrane region

8INI の概要

• エントリーDOI: 10.2210/pdb8ini/pdb
• 分子名称: vaccinia h2 protein, TETRAETHYLENE GLYCOL (3 entities in total)
• 機能のキーワード: membrane fusion, entry component, viral protein
• 由来する生物種: Vaccinia virus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 22804.13

構造登録者

Liu, C.Y.,Ko, T.P.,Wang, H.C.,Chang, W. (登録日: 2023-03-10, 公開日: 2023-11-15, 最終更新日: 2024-10-23)

主引用文献

Kao, C.F.,Liu, C.Y.,Hsieh, C.L.,Carillo, K.J.D.,Tzou, D.L.M.,Wang, H.C.,Chang, W.
Structural and functional analyses of viral H2 protein of the vaccinia virus entry fusion complex.
J.Virol., 97:e0134323-e0134323, 2023

PubMed Abstract: Vaccinia virus infection requires virus-cell membrane fusion to complete entry during endocytosis; however, it contains a large viral fusion protein complex of 11 viral proteins that share no structure or sequence homology to all the known viral fusion proteins, including type I, II, and III fusion proteins. It is thus very challenging to investigate how the vaccinia fusion complex works to trigger membrane fusion with host cells. In this study, we crystallized the ectodomain of vaccinia H2 protein, one component of the viral fusion complex. Furthermore, we performed a series of mutational, biochemical, and molecular analyses and identified two surface loops containing LGYSG and RRGTGDAW as the A28-binding region. We also showed that residues in the N-terminal helical region (amino acids 51-90) are also important for H2 function.

PubMed: 37975688
DOI: 10.1128/jvi.01343-23

実験手法

X-RAY DIFFRACTION (1.75 Å)