8IMW

Crystal structure of response regulator PmrA receiver domain

8IMW の概要

• エントリーDOI: 10.2210/pdb8imw/pdb
• 分子名称: Transcriptional regulator (2 entities in total)
• 機能のキーワード: polymyxin resistance, pmra, response regulator, receiver domain., dna binding protein
• 由来する生物種: Acinetobacter baumannii (strain ATCC 19606 / DSM 30007 / JCM 6841 / CCUG 19606 / CIP 70.34 / NBRC 109757 / NCIMB 12457 / NCTC 12156 / 81)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 30855.57

構造登録者

Ouyang, Z.,Wen, Y. (登録日: 2023-03-07, 公開日: 2024-03-13, 最終更新日: 2024-05-15)

主引用文献

Ouyang, Z.,He, W.,Jiao, M.,Yu, Q.,Guo, Y.,Refat, M.,Qin, Q.,Zhang, J.,Shi, Q.,Zheng, F.,Wen, Y.
Mechanistic and biophysical characterization of polymyxin resistance response regulator PmrA in Acinetobacter baumannii.
Front Microbiol, 15:1293990-1293990, 2024

PubMed Abstract: PmrAB is a crucial two-component regulatory system (TCS) that plays a vital role in conferring resistance to polymyxin. PmrA, a response regulator belonging to the OmpR/PhoB family, is composed of a C-terminal DNA-binding effector domain and an N-terminal receiver domain. The receiver domain can be phosphorylated by PmrB, a transmembrane sensor histidine kinase that interacts with PmrA. Once phosphorylated, PmrA undergoes a conformational change, resulting in the formation of a symmetric dimer in the receiver domain. This conformational change facilitates the recognition of promoter DNA by the DNA-binding domain of PmrA, leading to the activation of adaptive responses.

PubMed: 38476937
DOI: 10.3389/fmicb.2024.1293990

実験手法

X-RAY DIFFRACTION (1.6 Å)