8ILY

Crystal structure of the RRM domain of human SETD1A

8ILY の概要

• エントリーDOI: 10.2210/pdb8ily/pdb
• 分子名称: SET domain containing 1A, histone lysine methyltransferase (2 entities in total)
• 機能のキーワード: setd1a, rrm, structural protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 25283.37

構造登録者

Bao, S.,Xu, C. (登録日: 2023-03-05, 公開日: 2023-04-26, 最終更新日: 2024-05-29)

主引用文献

Bao, S.,Xu, C.
Molecular insight into the SETD1A/B N-terminal region and its interaction with WDR82.
Biochem.Biophys.Res.Commun., 658:136-140, 2023

PubMed Abstract: SETD1A and SETD1B originate from Set1, the sole H3K4 methyltransferase in yeast, and they play important roles in active gene transcription. Here, we present the crystal structures of the RRM domains of human SETD1A and SETD1B. Although both RRM domains adopt a canonical RRM fold, their structural features are different from that of the yeast Set1 RRM domain, their yeast homolog. By using an ITC binding assay, we found an intrinsically disordered region in SETD1A/B binds WDR82. The structural analysis implies that the positively charged regions within human RRM domains might be involved in binding to RNA. Our work provides structural insight into the assembly of WDR82 with the catalytic subunits SETD1A/B in the context of the whole complex.

PubMed: 37030068
DOI: 10.1016/j.bbrc.2023.03.064

実験手法

X-RAY DIFFRACTION (1.7 Å)