8ILQ

Structure of SFTSV Gn-Gc heterodimer

8ILQ の概要

• エントリーDOI: 10.2210/pdb8ilq/pdb
• EMDBエントリー: 35540
• 分子名称: Envelopment polyprotein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: sftsv, virion, icosahedral reconstruction, virus, viral protein
• 由来する生物種: Severe fever with thrombocytopenia syndrome virus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 118055.28

構造登録者

Du, S.,Peng, R.,Qi, J.,Li, C. (登録日: 2023-03-04, 公開日: 2023-09-13, 最終更新日: 2024-11-13)

主引用文献

Du, S.,Peng, R.,Xu, W.,Qu, X.,Wang, Y.,Wang, J.,Li, L.,Tian, M.,Guan, Y.,Wang, J.,Wang, G.,Li, H.,Deng, L.,Shi, X.,Ma, Y.,Liu, F.,Sun, M.,Wei, Z.,Jin, N.,Liu, W.,Qi, J.,Liu, Q.,Liao, M.,Li, C.
Cryo-EM structure of severe fever with thrombocytopenia syndrome virus.
Nat Commun, 14:6333-6333, 2023

PubMed Abstract: The severe fever with thrombocytopenia syndrome virus (SFTSV) is a tick-borne human-infecting bunyavirus, which utilizes two envelope glycoproteins, Gn and Gc, to enter host cells. However, the structure and organization of these glycoproteins on virion surface are not yet known. Here we describe the structure of SFTSV determined by single particle reconstruction, which allows mechanistic insights into bunyavirus assembly at near-atomic resolution. The SFTSV Gn and Gc proteins exist as heterodimers and further assemble into pentameric and hexameric peplomers, shielding the Gc fusion loops by both intra- and inter-heterodimer interactions. Individual peplomers are associated mainly through the ectodomains, in which the highly conserved glycans on N914 of Gc play a crucial role. This elaborate assembly stabilizes Gc in the metastable prefusion conformation and creates some cryptic epitopes that are only accessible in the intermediate states during virus entry. These findings provide an important basis for developing vaccines and therapeutic drugs.

PubMed: 37816705
DOI: 10.1038/s41467-023-41804-7

実験手法

ELECTRON MICROSCOPY (4.3 Å)