8ILK

Crystal structure of a highly photostable and bright green fluorescent protein at pH8.5

8ILK の概要

• エントリーDOI: 10.2210/pdb8ilk/pdb
• 分子名称: Green FLUORESCENT PROTEIN, CHLORIDE ION (3 entities in total)
• 機能のキーワード: photostable, green fluorescent protein, fluorescent protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 50751.49

構造登録者

Ago, H.,Ando, R.,Hirano, M.,Shimozono, S.,Miyawaki, A.,Yamamoto, M. (登録日: 2023-03-03, 公開日: 2023-10-04, 最終更新日: 2024-10-30)

主引用文献

Ando, R.,Shimozono, S.,Ago, H.,Takagi, M.,Sugiyama, M.,Kurokawa, H.,Hirano, M.,Niino, Y.,Ueno, G.,Ishidate, F.,Fujiwara, T.,Okada, Y.,Yamamoto, M.,Miyawaki, A.
StayGold variants for molecular fusion and membrane-targeting applications.
Nat.Methods, 21:648-656, 2024

PubMed Abstract: Although StayGold is a bright and highly photostable fluorescent protein, its propensity for obligate dimer formation may hinder applications in molecular fusion and membrane targeting. To attain monovalent as well as bright and photostable labeling, we engineered tandem dimers of StayGold to promote dispersibility. On the basis of the crystal structure of this fluorescent protein, we disrupted the dimerization to generate a monomeric variant that offers improved photostability and brightness compared to StayGold. We applied the new monovalent StayGold tools to live-cell imaging experiments using spinning-disk laser-scanning confocal microscopy or structured illumination microscopy. We achieved cell-wide, high-spatiotemporal resolution and sustained imaging of dynamic subcellular events, including the targeting of endogenous condensin I to mitotic chromosomes, the movement of the Golgi apparatus and its membranous derivatives along microtubule networks, the distribution of cortical filamentous actin and the remolding of cristae membranes within mobile mitochondria.

PubMed: 38036853
DOI: 10.1038/s41592-023-02085-6

実験手法

X-RAY DIFFRACTION (1.56 Å)