8IE3

human nuclear pre-60S ribosomal particle - State E

これはPDB形式変換不可エントリーです。

8IE3 の概要

• エントリーDOI: 10.2210/pdb8ie3/pdb
• EMDBエントリー: 35375
• 分子名称: 5S rRNA, 60S ribosomal protein L34, 60S ribosomal protein L7a, ... (48 entities in total)
• 機能のキーワード: gnl2, nuclear, pre-60s, ribosome
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 48
• 化学式量合計: 2773273.19

構造登録者

Zhang, Y.,Gao, N. (登録日: 2023-02-15, 公開日: 2023-08-09, 最終更新日: 2023-11-15)

主引用文献

Zhang, Y.,Liang, X.,Luo, S.,Chen, Y.,Li, Y.,Ma, C.,Li, N.,Gao, N.
Visualizing the nucleoplasmic maturation of human pre-60S ribosomal particles.
Cell Res., 33:867-878, 2023

PubMed Abstract: Eukaryotic ribosome assembly is a highly orchestrated process that involves over two hundred protein factors. After early assembly events on nascent rRNA in the nucleolus, pre-60S particles undergo continuous maturation steps in the nucleoplasm, and prepare for nuclear export. Here, we report eleven cryo-EM structures of the nuclear pre-60S particles isolated from human cells through epitope-tagged GNL2, at resolutions of 2.8-4.3 Å. These high-resolution snapshots provide fine details for several major structural remodeling events at a virtual temporal resolution. Two new human nuclear factors, L10K and C11orf98, were also identified. Comparative structural analyses reveal that many assembly factors act as successive place holders to control the timing of factor association/dissociation events. They display multi-phasic binding properties for different domains and generate complex binding inter-dependencies as a means to guide the rRNA maturation process towards its mature conformation. Overall, our data reveal that nuclear assembly of human pre-60S particles is generally hierarchical with short branch pathways, and a few factors display specific roles as rRNA chaperones by confining rRNA helices locally to facilitate their folding, such as the C-terminal domain of SDAD1.

PubMed: 37491604
DOI: 10.1038/s41422-023-00853-9

実験手法

ELECTRON MICROSCOPY (3.3 Å)