8IBL

MES bound form of PET-degrading cutinase Cut190 with thermostability-improving mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H and S176A inactivation

8IBL の概要

• エントリーDOI: 10.2210/pdb8ibl/pdb
• 分子名称: Alpha/beta hydrolase family protein, CALCIUM ION, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, ... (5 entities in total)
• 機能のキーワード: protein engineering, polyesterase, disulfide bond, metal binding, ligand complex, hydrolase
• 由来する生物種: Saccharomonospora viridis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 58320.61

構造登録者

Emori, M.,Numoto, N.,Kamiya, N.,Oda, M. (登録日: 2023-02-10, 公開日: 2023-03-15)

• 主引用文献: Numoto, N.,Kamiya, N.,Oda, M.; Improvement of thermostability and activity of PET-degrading enzyme Cut190 towards a detailed understanding and application of the enzymatic reaction mechanism.; Biorxiv, 2023; DOI: 10.1101/2023.02.26.529345

実験手法

X-RAY DIFFRACTION (2.6 Å)