8IBI

Inactive mutant of CtPL-H210S/F214I

8IBI の概要

• エントリーDOI: 10.2210/pdb8ibi/pdb
• 分子名称: PET hydrolase, 2-acetamido-2-deoxy-beta-D-glucopyranose, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: pet hydrolase, alpha/beta hydrolase, caldimonas taiwanensis, hydrolase
• 由来する生物種: Caldimonas taiwanensis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 58928.67

構造登録者

Li, X.,Shi, B.L.,Zeng, Z.Y.,Huang, J.-W.,Chen, C.-C.,Guo, R.-T. (登録日: 2023-02-10, 公開日: 2023-04-19, 最終更新日: 2024-11-20)

主引用文献

Li, X.,Shi, B.,Huang, J.W.,Zeng, Z.,Yang, Y.,Zhang, L.,Min, J.,Chen, C.C.,Guo, R.T.
Functional tailoring of a PET hydrolytic enzyme expressed in Pichia pastoris.
Bioresour Bioprocess, 10:26-26, 2023

PubMed Abstract: Using enzymes to hydrolyze and recycle poly(ethylene terephthalate) (PET) is an attractive eco-friendly approach to manage the ever-increasing PET wastes, while one major challenge to realize the commercial application of enzyme-based PET degradation is to establish large-scale production methods to produce PET hydrolytic enzyme. To achieve this goal, we exploited the industrial strain Pichia pastoris to express a PET hydrolytic enzyme from Caldimonas taiwanensis termed CtPL-DM. In contrast to the protein expressed in Escherichia coli, CtPL-DM expressed in P. pastoris is inactive in PET degradation. Structural analysis indicates that a putative N-glycosylation site N181 could restrain the conformational change of a substrate-binding Trp and hamper the enzyme action. We thus constructed N181A to remove the N-glycosylation and found that the PET hydrolytic activity of this variant was restored. The performance of N181A was further enhanced via molecular engineering. These results are of valuable in terms of PET hydrolytic enzyme production in industrial strains in the future.

PubMed: 38647782
DOI: 10.1186/s40643-023-00648-1

実験手法

X-RAY DIFFRACTION (2.14 Å)