8IA1

plastidial glycerol-3-phosphate acyltransferases (GPAT) from the green alga Myrmecia incisa

8IA1 の概要

• エントリーDOI: 10.2210/pdb8ia1/pdb
• 分子名称: Glycerol-3-phosphate acyltransferase, chloroplastic (2 entities in total)
• 機能のキーワード: plastidial glycerol-3-phosphate acyltransferases, transferase
• 由来する生物種: Lobosphaera incisa
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 124953.54

構造登録者

Song, X. (登録日: 2023-02-07, 公開日: 2024-02-07, 最終更新日: 2025-02-19)

主引用文献

Li, X.,Yang, M.,Sun, D.,Shi, J.,Yang, M.,Feng, Y.,Xue, S.
Unique recognition of the microalgal plastidial glycerol-3-phosphate acyltransferase for acyl-ACP.
Plant Sci., 332:111725-111725, 2023

PubMed Abstract: Plastidial glycerol-3-phosphate acyltransferases (GPATs) catalyze acyl-ACP and glycerol-3-phosphate to synthesize lysophosphatidic acid in vivo, which initiates the formation of various glycerolipids. Although the physiological substrates of plastidial GPATs are acyl-ACPs, acyl-CoAs have been commonly studied on the GPATs in vitro. However, little is known whether there are any distinct features of GPATs towards acyl-ACP and acyl-CoA. In this study, the results showed that the microalgal plastidial GPATs preferred acyl-ACP to acyl-CoA, while surprisingly, the plant-derived plastidial GPATs showed no obvious preferences towards these two acyl carriers. The key residues responsible for the distinct feature of microalgal plastidial GPATs were compared with plant-derived plastidial GPATs in their efficiency to catalyze acyl-ACP and acyl-CoA. Microalgal plastidial GPATs uniquely recognized acyl-ACP as compared to with other acyltransferases. The structure of the acyltransferases-ACP complex highlights only the involvement of the large structural domain in ACP in microalgal plastidial GPAT while in the other acyltransferases, both large and small structural domains were involved in the recognition process. The interaction sites on the plastidial GPAT from the green alga Myrmecia incisa (MiGPAT1) with ACP turned out to be K204, R212 and R266. A unique recognition between the microalgal plastidial GPAT and ACP was elucidated.

PubMed: 37142097
DOI: 10.1016/j.plantsci.2023.111725

実験手法

X-RAY DIFFRACTION (2.31 Å)