8I9E

S-RBD(Omicron BA.3) in complex with PD of ACE2

8I9E の概要

• エントリーDOI: 10.2210/pdb8i9e/pdb
• EMDBエントリー: 35268
• 分子名称: Processed angiotensin-converting enzyme 2, Spike protein S1, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: sars-cov-2, viral protein, hydrolase-viral protein complex, hydrolase/viral protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 99229.52

構造登録者

Li, Y.N.,Shen, Y.P.,Zhang, Y.Y.,Yan, R.H. (登録日: 2023-02-06, 公開日: 2024-02-07, 最終更新日: 2024-10-23)

主引用文献

Li, Y.,Shen, Y.,Zhang, Y.,Yan, R.
Structural Basis for the Enhanced Infectivity and Immune Evasion of Omicron Subvariants.
Viruses, 15:-, 2023

PubMed Abstract: The Omicron variants of SARS-CoV-2 have emerged as the dominant strains worldwide, causing the COVID-19 pandemic. Each Omicron subvariant contains at least 30 mutations on the spike protein (S protein) compared to the original wild-type (WT) strain. Here we report the cryo-EM structures of the trimeric S proteins from the BA.1, BA.2, BA.3, and BA.4/BA.5 subvariants, with BA.4 and BA.5 sharing the same S protein mutations, each in complex with the surface receptor ACE2. All three receptor-binding domains of the S protein from BA.2 and BA.4/BA.5 are "up", while the BA.1 S protein has two "up" and one "down". The BA.3 S protein displays increased heterogeneity, with the majority in the all "up" RBD state. The different conformations preferences of the S protein are consistent with their varied transmissibility. By analyzing the position of the glycan modification on Asn343, which is located at the S309 epitopes, we have uncovered the underlying immune evasion mechanism of the Omicron subvariants. Our findings provide a molecular basis of high infectivity and immune evasion of Omicron subvariants, thereby offering insights into potential therapeutic interventions against SARS-CoV-2 variants.

PubMed: 37376697
DOI: 10.3390/v15061398

実験手法

ELECTRON MICROSCOPY (3.2 Å)