8I8G

Crystal structure of Cph001-D189N in complex with CMN IIA and ATP

8I8G の概要

• エントリーDOI: 10.2210/pdb8i8g/pdb
• 関連するBIRD辞書のPRD_ID: PRD_000193
• 分子名称: Viomycin kinase, KBE-DPP-UAL-MYN-DPP-SER, ADENOSINE-5'-TRIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: phosphotransferase, capreomycin, cph, resistance, transferase, atp
• 由来する生物種: Streptosporangium roseum; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 62071.13

構造登録者

Chang, C.Y.,Toh, S.I.,Elaine K, J.,Hsiao, P.Y. (登録日: 2023-02-04, 公開日: 2024-01-10)

主引用文献

Toh, S.I.,Elaine Keisha, J.,Wang, Y.L.,Pan, Y.C.,Jhu, Y.H.,Hsiao, P.Y.,Liao, W.T.,Chen, P.Y.,Ko, T.M.,Chang, C.Y.
Discovery and characterization of genes conferring natural resistance to the antituberculosis antibiotic capreomycin.
Commun Biol, 6:1282-1282, 2023

PubMed Abstract: Metagenomic-based studies have predicted an extraordinary number of potential antibiotic-resistance genes (ARGs). These ARGs are hidden in various environmental bacteria and may become a latent crisis for antibiotic therapy via horizontal gene transfer. In this study, we focus on a resistance gene cph, which encodes a phosphotransferase (Cph) that confers resistance to the antituberculosis drug capreomycin (CMN). Sequence Similarity Network (SSN) analysis classified 353 Cph homologues into five major clusters, where the proteins in cluster I were found in a broad range of actinobacteria. We examine the function and antibiotics targeted by three putative resistance proteins in cluster I via biochemical and protein structural analysis. Our findings reveal that these three proteins in cluster I confer resistance to CMN, highlighting an important aspect of CMN resistance within this gene family. This study contributes towards understanding the sequence-structure-function relationships of the phosphorylation resistance genes that confer resistance to CMN.

PubMed: 38114770
DOI: 10.1038/s42003-023-05681-6

実験手法

X-RAY DIFFRACTION (3 Å)