8I7R

In situ structure of axonemal doublet microtubules in mouse sperm with 48-nm repeat

これはPDB形式変換不可エントリーです。

8I7R の概要

• エントリーDOI: 10.2210/pdb8i7r/pdb
• EMDBエントリー: 35222 35224 35225 35226 35227 35228 35230
• 分子名称: Meiosis-specific nuclear structural protein 1, Tektin bundle-interacting protein 1, Tektin-5, ... (39 entities in total)
• 機能のキーワード: microtubules, axoneme, sperm, filament, structural protein
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 450
• 化学式量合計: 21412304.33

構造登録者

Zhu, Y.,Yin, G.L.,Tai, L.H.,Sun, F. (登録日: 2023-02-02, 公開日: 2023-10-11, 最終更新日: 2025-09-17)

主引用文献

Tai, L.,Yin, G.,Huang, X.,Sun, F.,Zhu, Y.
In-cell structural insight into the stability of sperm microtubule doublet.
Cell Discov, 9:116-116, 2023

PubMed Abstract: The propulsion for mammalian sperm swimming is generated by flagella beating. Microtubule doublets (DMTs) along with microtubule inner proteins (MIPs) are essential structural blocks of flagella. However, the intricate molecular architecture of intact sperm DMT remains elusive. Here, by in situ cryo-electron tomography, we solved the in-cell structure of mouse sperm DMT at 4.5-7.5 Å resolutions, and built its model with 36 kinds of MIPs in 48 nm periodicity. We identified multiple copies of Tektin5 that reinforce Tektin bundle, and multiple MIPs with different periodicities that anchor the Tektin bundle to tubulin wall. This architecture contributes to a superior stability of A-tubule than B-tubule of DMT, which was revealed by structural comparison of DMTs from the intact and deformed axonemes. Our work provides an overall molecular picture of intact sperm DMT in 48 nm periodicity that is essential to understand the molecular mechanism of sperm motility as well as the related ciliopathies.

PubMed: 37989994
DOI: 10.1038/s41421-023-00606-3

実験手法

ELECTRON MICROSCOPY (6.5 Å)