8I4N

Crystal strcuture of 6-phosphogluconate dehydrogenase from Corynebacterium glutamicum

8I4N の概要

• エントリーDOI: 10.2210/pdb8i4n/pdb
• 分子名称: 6-phosphogluconate dehydrogenase, decarboxylating (2 entities in total)
• 機能のキーワード: nadp dependent enzyme, oxidoreductase
• 由来する生物種: Corynebacterium glutamicum ATCC 13032
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 105709.94

構造登録者

Yu, H.,Kim, K.-J. (登録日: 2023-01-20, 公開日: 2023-07-26, 最終更新日: 2024-05-29)

主引用文献

Yu, H.,Hong, J.,Seok, J.,Seu, Y.B.,Kim, I.K.,Kim, K.J.
Crystal Structures of 6-Phosphogluconate Dehydrogenase from Corynebacterium glutamicum.
J Microbiol Biotechnol., 33:1-9, 2023

PubMed Abstract: () has been considered a very important and meaningful industrial microorganism for the production of amino acids worldwide. To produce amino acids, cells require nicotinamide adenine dinucleotide phosphate (NADPH), which is a biological reducing agent. The pentose phosphate pathway (PPP) can supply NADPH in cells via the 6-phosphogluconate dehydrogenase (6PGD) enzyme, which is an oxidoreductase that converts 6-phosphogluconate (6PG) to ribulose 5-phosphate (Ru5P), to produce NADPH. In this study, we identified the crystal structure of 6PGD_apo and 6PGD_NADP from ATCC 13032 (6PGD) and reported our biological research based on this structure. We identified the substrate binding site and co-factor binding site of 6PGD, which are crucial for understanding this enzyme. Based on the findings of our research, 6PGD is expected to be used as a NADPH resource in the food industry and as a drug target in the pharmaceutical industry.

PubMed: 37417004
DOI: 10.4014/jmb.2305.05002

実験手法

X-RAY DIFFRACTION (2.41 Å)