8I3E

Crystal structure of ELKS1 in complex with Piccolo

Summary for 8I3E

• Entry DOI: 10.2210/pdb8i3e/pdb
• Descriptor: ELKS/Rab6-interacting/CAST family member 1, MKIAA0559 protein (2 entities in total)
• Functional Keywords: elks, piccolo, scaffold protein, presynapse, synaptic vesicle, protein binding
• Biological source: Rattus norvegicus (Norway rat); More
• Total number of polymer chains: 3
• Total formula weight: 44376.21

Authors

Cai, Q.,Zhang, M. (deposition date: 2023-01-17, release date: 2024-01-24, Last modification date: 2024-05-22)

Primary citation

Qiu, H.,Wu, X.,Ma, X.,Li, S.,Cai, Q.,Ganzella, M.,Ge, L.,Zhang, H.,Zhang, M.
Short-distance vesicle transport via phase separation.
Cell, 187:2175-2193.e21, 2024

PubMed Abstract: In addition to long-distance molecular motor-mediated transport, cellular vesicles also need to be moved at short distances with defined directions to meet functional needs in subcellular compartments but with unknown mechanisms. Such short-distance vesicle transport does not involve molecular motors. Here, we demonstrate, using synaptic vesicle (SV) transport as a paradigm, that phase separation of synaptic proteins with vesicles can facilitate regulated, directional vesicle transport between different presynaptic bouton sub-compartments. Specifically, a large coiled-coil scaffold protein Piccolo, in response to Ca and via its C2A domain-mediated Ca sensing, can extract SVs from the synapsin-clustered reserve pool condensate and deposit the extracted SVs onto the surface of the active zone protein condensate. We further show that the Trk-fused gene, TFG, also participates in COPII vesicle trafficking from ER to the ER-Golgi intermediate compartment via phase separation. Thus, phase separation may play a general role in short-distance, directional vesicle transport in cells.

PubMed: 38552623
DOI: 10.1016/j.cell.2024.03.003

Experimental method

X-RAY DIFFRACTION (2.7 Å)