8I3C

Cryo-EM structure of abscisic acid transporter AtABCG25 with CHS

8I3C の概要

• エントリーDOI: 10.2210/pdb8i3c/pdb
• EMDBエントリー: 35149
• 分子名称: ABC transporter G family member 25, CHOLESTEROL HEMISUCCINATE (2 entities in total)
• 機能のキーワード: transport, abc transporter, plant hormone, membrane protein
• 由来する生物種: Arabidopsis thaliana (thale cress)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 146941.19

構造登録者

Huang, X.,Zhang, X.,Zhang, P. (登録日: 2023-01-16, 公開日: 2023-09-13, 最終更新日: 2023-11-01)

主引用文献

Huang, X.,Zhang, X.,An, N.,Zhang, M.,Ma, M.,Yang, Y.,Jing, L.,Wang, Y.,Chen, Z.,Zhang, P.
Cryo-EM structure and molecular mechanism of abscisic acid transporter ABCG25.
Nat.Plants, 9:1709-1719, 2023

PubMed Abstract: Abscisic acid (ABA) is one of the plant hormones that regulate various physiological processes, including stomatal closure, seed germination and development. ABA is synthesized mainly in vascular tissues and transported to distal sites to exert its physiological functions. Many ABA transporters have been identified, however, the molecular mechanism of ABA transport remains elusive. Here we report the cryogenic electron microscopy structure of the Arabidopsis thaliana adenosine triphosphate-binding cassette G subfamily ABA exporter ABCG25 (AtABCG25) in inward-facing apo conformation, ABA-bound pre-translocation conformation and outward-facing occluded conformation. Structural and biochemical analyses reveal that the ABA bound with ABCG25 adopts a similar configuration as that in ABA receptors and that the ABA-specific binding is dictated by residues from transmembrane helices TM1, TM2 and TM5a of each protomer at the transmembrane domain interface. Comparison of different conformational structures reveals conformational changes, especially those of transmembrane helices and residues constituting the substrate translocation pathway during the cross-membrane transport process. Based on the structural data, a 'gate-flipper' translocation model of ABCG25-mediated ABA cross-membrane transport is proposed. Our structural data on AtABCG25 provide new clues to the physiological study of ABA and shed light on its potential applications in plants and agriculture.

PubMed: 37666961
DOI: 10.1038/s41477-023-01509-7

実験手法

ELECTRON MICROSCOPY (2.85 Å)