8I26

NMR structure of Toxoplasma gondii PDCD5 (cis form)

8I26 の概要

• エントリーDOI: 10.2210/pdb8i26/pdb
• 分子名称: Programmed cell death 5 protein (1 entity in total)
• 機能のキーワード: molten globule, heparan sulfate/heparin binding protein, proline cis form, endocytosis
• 由来する生物種: Toxoplasma gondii GT1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13716.47

構造登録者

Lin, M.H.,Hsu, C.H. (登録日: 2023-01-14, 公開日: 2024-01-17, 最終更新日: 2024-06-12)

主引用文献

Lin, G.M.,Yu, T.A.,Chang, C.F.,Hsu, C.H.
Proline Isomerization and Molten Globular Property of TgPDCD5 Secreted from Toxoplasma gondii Confers Its Regulation of Heparin Sulfate Binding.
Jacs Au, 4:1763-1774, 2024

PubMed Abstract: Toxoplasmosis, caused by , poses risks to vulnerable populations. TgPDCD5, a secreted protein of , induces apoptosis through heparan sulfate-mediated endocytosis. The entry mechanism of TgPDCD5 has remained elusive. Here, we present the solution structure of TgPDCD5 as a helical bundle with an extended N-terminal helix, exhibiting molten globule characteristics. NMR perturbation studies reveal heparin/heparan sulfate binding involving the heparan sulfate/heparin proteoglycans-binding motif and the core region, influenced by proline isomerization of P107 residue. The heterogeneous proline recruits a cyclophilin TgCyp18, accelerating interconversion between conformers and regulating heparan/heparin binding. These atomic-level insights elucidate the binary switch's functionality, expose novel heparan sulfate-binding surfaces, and illuminate the unconventional cellular entry of pathogenic TgPDCD5.

PubMed: 38818051
DOI: 10.1021/jacsau.3c00577

実験手法

SOLUTION NMR