8I24

Clostridium thermocellum RNA polymerase transcription open complex with SigI6 and its promoter

8I24 の概要

• エントリーDOI: 10.2210/pdb8i24/pdb
• 関連するPDBエントリー: 8I23
• EMDBエントリー: 35131
• 分子名称: DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (9 entities in total)
• 機能のキーワード: transcription open complex, sigi, transcription
• 由来する生物種: Acetivibrio thermocellus DSM 1313; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 431383.80

構造登録者

Li, J.,Zhang, H.,Li, D.,Feng, Y.,Zhu, P. (登録日: 2023-01-13, 公開日: 2023-10-11, 最終更新日: 2023-11-01)

主引用文献

Li, J.,Zhang, H.,Li, D.,Liu, Y.J.,Bayer, E.A.,Cui, Q.,Feng, Y.,Zhu, P.
Structure of the transcription open complex of distinct sigma I factors.
Nat Commun, 14:6455-6455, 2023

PubMed Abstract: Bacterial σ factors of the σ-family are widespread in Bacilli and Clostridia and are involved in the heat shock response, iron metabolism, virulence, and carbohydrate sensing. A multiplicity of σ paralogues in some cellulolytic bacteria have been shown to be responsible for the regulation of the cellulosome, a multienzyme complex that mediates efficient cellulose degradation. Here, we report two structures at 3.0 Å and 3.3 Å of two transcription open complexes formed by two σ factors, SigI1 and SigI6, respectively, from the thermophilic, cellulolytic bacterium, Clostridium thermocellum. These structures reveal a unique, hitherto-unknown recognition mode of bacterial transcriptional promoters, both with respect to domain organization and binding to promoter DNA. The key characteristics that determine the specificities of the σ paralogues were further revealed by comparison of the two structures. Consequently, the σ factors represent a distinct set of the σ-family σ factors, thus highlighting the diversity of bacterial transcription.

PubMed: 37833284
DOI: 10.1038/s41467-023-41796-4

実験手法

ELECTRON MICROSCOPY (3.36 Å)