8I0P

The cryo-EM structure of human pre-Bact complex

これはPDB形式変換不可エントリーです。

8I0P の概要

• エントリーDOI: 10.2210/pdb8i0p/pdb
• EMDBエントリー: 35105
• 分子名称: Pre-mRNA-processing-splicing factor 8, Crooked neck-like protein 1, DNA/RNA-binding protein KIN17, ... (47 entities in total)
• 機能のキーワード: spliceosome, pre-bact complex, rna splicing, prp2, activation, splicing
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 51
• 化学式量合計: 2845662.38

構造登録者

Zhan, X.,Lu, Y.,Shi, Y. (登録日: 2023-01-11, 公開日: 2024-07-31, 最終更新日: 2025-06-25)

主引用文献

Zhan, X.,Lu, Y.,Shi, Y.
Molecular basis for the activation of human spliceosome.
Nat Commun, 15:6348-6348, 2024

PubMed Abstract: The spliceosome executes pre-mRNA splicing through four sequential stages: assembly, activation, catalysis, and disassembly. Activation of the spliceosome, namely remodeling of the pre-catalytic spliceosome (B complex) into the activated spliceosome (B complex) and the catalytically activated spliceosome (B complex), involves major flux of protein components and structural rearrangements. Relying on a splicing inhibitor, we have captured six intermediate states between the B and B complexes: pre-B, B-I, B-II, B-III, B-IV, and post-B. Their cryo-EM structures, together with an improved structure of the catalytic step I spliceosome (C complex), reveal how the catalytic center matures around the internal stem loop of U6 snRNA, how the branch site approaches 5'-splice site, how the RNA helicase PRP2 rearranges to bind pre-mRNA, and how U2 snRNP undergoes remarkable movement to facilitate activation. We identify a previously unrecognized key role of PRP2 in spliceosome activation. Our study recapitulates a molecular choreography of the human spliceosome during its catalytic activation.

PubMed: 39068178
DOI: 10.1038/s41467-024-50785-0

実験手法

ELECTRON MICROSCOPY (3.4 Å)