8HTW

Crystal Structure of the ring nuclease Sso2081 Y133F mutant from Saccharolobus solfataricus in its apo form

8HTW の概要

• エントリーDOI: 10.2210/pdb8htw/pdb
• 分子名称: CRISPR system ring nuclease SSO2081 (2 entities in total)
• 機能のキーワード: sso2081, ring nuclease, mutant, y133f, hydrolase
• 由来する生物種: Saccharolobus solfataricus P2
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 41077.68

構造登録者

Lin, Z.,Du, L.,Luo, Z. (登録日: 2022-12-21, 公開日: 2023-02-15, 最終更新日: 2024-10-16)

主引用文献

Du, L.,Zhang, D.,Luo, Z.,Lin, Z.
Molecular basis of stepwise cyclic tetra-adenylate cleavage by the type III CRISPR ring nuclease Crn1/Sso2081.
Nucleic Acids Res., 51:2485-2495, 2023

PubMed Abstract: The cyclic oligoadenylates (cOAs) act as second messengers of the type III CRISPR immunity system through activating the auxiliary nucleases for indiscriminate RNA degradation. The cOA-degrading nucleases (ring nucleases) provide an 'off-switch' regulation of the signaling, thereby preventing cell dormancy or cell death. Here, we describe the crystal structures of the founding member of CRISPR-associated ring nuclease 1 (Crn1) Sso2081 from Saccharolobus solfataricus, alone, bound to phosphate ions or cA4 in both pre-cleavage and cleavage intermediate states. These structures together with biochemical characterizations establish the molecular basis of cA4 recognition and catalysis by Sso2081. The conformational changes in the C-terminal helical insert upon the binding of phosphate ions or cA4 reveal a gate-locking mechanism for ligand binding. The critical residues and motifs identified in this study provide a new insight to distinguish between cOA-degrading and -nondegrading CARF domain-containing proteins.

PubMed: 36807980
DOI: 10.1093/nar/gkad101

実験手法

X-RAY DIFFRACTION (2 Å)