8HPD

Bry-LHCII heterotrimer of Bryopsis corticulans

8HPD の概要

• エントリーDOI: 10.2210/pdb8hpd/pdb
• EMDBエントリー: 34930
• 分子名称: siphonaxanthin chlorophyll a/b binding light-harvesting complex II, Bry-Lhcb1, siphonaxanthin chlorophyll a/b binding light-harvesting complex II, Bry-Lhcb3, siphonaxanthin chlorophyll a/b binding light-harvesting complex II, Bry-Lhcb2, ... (9 entities in total)
• 機能のキーワード: light-harvesting complexes, siphonaxanthin, siphonein, photosynthesis
• 由来する生物種: Bryopsis corticulans; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 129098.05

構造登録者

Li, Z.H.,Shen, J.R.,Wang, W.D. (登録日: 2022-12-12, 公開日: 2023-09-06, 最終更新日: 2025-07-02)

主引用文献

Li, Z.,Zhou, C.,Zhao, S.,Zhang, J.,Liu, X.,Sang, M.,Qin, X.,Yang, Y.,Han, G.,Kuang, T.,Shen, J.R.,Wang, W.
Structural and functional properties of different types of siphonous LHCII trimers from an intertidal green alga Bryopsis corticulans.
Structure, 31:1247-1258.e3, 2023

PubMed Abstract: Light-harvesting complexes of photosystem II (LHCIIs) in green algae and plants are vital antenna apparatus for light harvesting, energy transfer, and photoprotection. Here we determined the structure of a siphonous-type LHCII trimer from the intertidal green alga Bryopsis corticulans by X-ray crystallography and cryo-electron microscopy (cryo-EM), and analyzed its functional properties by spectral analysis. The Bryopsis LHCII (Bry-LHCII) structures in both homotrimeric and heterotrimeric form show that green light-absorbing siphonaxanthin and siphonein occupied the sites of lutein and violaxanthin in plant LHCII, and two extra chlorophylls (Chls) b replaced Chls a. Binding of these pigments expands the blue-green light absorption of B. corticulans in the tidal zone. We observed differences between the Bry-LHCII homotrimer crystal and cryo-EM structures, and also between Bry-LHCII homotrimer and heterotrimer cryo-EM structures. These conformational changes may reflect the flexibility of Bry-LHCII, which may be required to adapt to light fluctuations from tidal rhythms.

PubMed: 37633266
DOI: 10.1016/j.str.2023.08.001

実験手法

ELECTRON MICROSCOPY (2.74 Å)