8HO3

Capsid of DT57C bacteriophage in the full state

Summary for 8HO3

• Entry DOI: 10.2210/pdb8ho3/pdb
• EMDB information: 34920
• Descriptor: Major head protein (1 entity in total)
• Functional Keywords: capsid, t5, mhp, virus
• Biological source: Escherichia phage DT57C
• Total number of polymer chains: 13
• Total formula weight: 658988.17

Authors

Ayala, R.,Moiseenko, A.V.,Chen, T.H.,Kulikov, E.E.,Golomidova, A.K.,Orekhov, P.S.,Street, M.A.,Sokolova, O.S.,Letarov, A.V.,Wolf, M. (deposition date: 2022-12-09, release date: 2023-12-13, Last modification date: 2023-12-27)

Primary citation

Ayala, R.,Moiseenko, A.V.,Chen, T.H.,Kulikov, E.E.,Golomidova, A.K.,Orekhov, P.S.,Street, M.A.,Sokolova, O.S.,Letarov, A.V.,Wolf, M.
Nearly complete structure of bacteriophage DT57C reveals architecture of head-to-tail interface and lateral tail fibers.
Nat Commun, 14:8205-8205, 2023

PubMed Abstract: The T5 family of viruses are tailed bacteriophages characterized by a long non-contractile tail. The bacteriophage DT57C is closely related to the paradigmal T5 phage, though it recognizes a different receptor (BtuB) and features highly divergent lateral tail fibers (LTF). Considerable portions of T5-like phages remain structurally uncharacterized. Here, we present the structure of DT57C determined by cryo-EM, and an atomic model of the virus, which was further explored using all-atom molecular dynamics simulations. The structure revealed a unique way of LTF attachment assisted by a dodecameric collar protein LtfC, and an unusual composition of the phage neck constructed of three protein rings. The tape measure protein (TMP) is organized within the tail tube in a three-stranded parallel α-helical coiled coil which makes direct contact with the genomic DNA. The presence of the C-terminal fragment of the TMP that remains within the tail tip suggests that the tail tip complex returns to its original state after DNA ejection. Our results provide a complete atomic structure of a T5-like phage, provide insights into the process of DNA ejection as well as a structural basis for the design of engineered phages and future mechanistic studies.

PubMed: 38081816
DOI: 10.1038/s41467-023-43824-9

Experimental method

ELECTRON MICROSCOPY (2.9 Å)