8HN2

Selenomethionine-labelled soluble domain of Rieske iron-sulfur protein from chlorobaculum tepidum

8HN2 の概要

• エントリーDOI: 10.2210/pdb8hn2/pdb
• NMR情報: BMRB: 51723
• 分子名称: Cytochrome b6-f complex iron-sulfur subunit, FE2/S2 (INORGANIC) CLUSTER (3 entities in total)
• 機能のキーワード: rieske iron-sulfur protein, electron transport, photosynthesis, green sulfur bacteria, cytochrome bc complex
• 由来する生物種: Chlorobaculum tepidum
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 23690.51

構造登録者

Kishimoto, H.,Mutoh, R.,Tanaka, H.,Kurisu, G.,Oh-oka, H. (登録日: 2022-12-07, 公開日: 2023-07-05, 最終更新日: 2024-11-13)

主引用文献

Kishimoto, H.,Azai, C.,Yamamoto, T.,Mutoh, R.,Nakaniwa, T.,Tanaka, H.,Miyanoiri, Y.,Kurisu, G.,Oh-Oka, H.
Soluble domains of cytochrome c-556 and Rieske iron-sulfur protein from Chlorobaculum tepidum: Crystal structures and interaction analysis.
Curr Res Struct Biol, 5:100101-100101, 2023

PubMed Abstract: In photosynthetic green sulfur bacteria, the electron transfer reaction from menaquinol:cytochrome oxidoreductase to the P840 reaction center (RC) complex occurs directly without any involvement of soluble electron carrier protein(s). X-ray crystallography has determined the three-dimensional structures of the soluble domains of the gene product and Rieske iron-sulfur protein (ISP). The former is a mono-heme cytochrome with an α-absorption peak at 556 nm. The overall fold of the soluble domain of cytochrome -556 (designated as cyt -556) consists of four α-helices and is very similar to that of water-soluble cyt -554 that independently functions as an electron donor to the P840 RC complex. However, the latter's remarkably long and flexible loop between the α3 and α4 helices seems to make it impossible to be a substitute for the former. The structure of the soluble domain of the Rieske ISP (Rieske protein) shows a typical β-sheets-dominated fold with a small cluster-binding and a large subdomain. The architecture of the Rieske protein is bilobal and belongs to those of -type Rieske ISPs. Nuclear magnetic resonance (NMR) measurements revealed weak non-polar but specific interaction sites on Rieske protein when mixed with cyt -556. Therefore, menaquinol:cytochrome oxidoreductase in green sulfur bacteria features a Rieske/cyt complex tightly associated with membrane-anchored cyt -556.

PubMed: 37180033
DOI: 10.1016/j.crstbi.2023.100101

実験手法

X-RAY DIFFRACTION (2.3 Å)