8HKA

TPA bound-form of Periplasmic terephthalate binding protein (TBP) from Ideonella sakaiensis

8HKA の概要

• エントリーDOI: 10.2210/pdb8hka/pdb
• 分子名称: Periplasmic terephthalate binding protein (TBP), terephthalic acid (3 entities in total)
• 機能のキーワード: periplasmic protein, terephthalate binding protein, transportation protein, ideonella sakaiensis, transport protein
• 由来する生物種: Ideonella sakaiensis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 33237.76

構造登録者

Lee, S.H.,Seo, H.,Kim, K.-J. (登録日: 2022-11-25, 公開日: 2023-06-21, 最終更新日: 2024-05-22)

主引用文献

Lee, S.H.,Seo, H.,Hong, H.,Kim, M.,Kim, K.J.
Molecular mechanism underlying high-affinity terephthalate binding and conformational change of TBP from Ideonella sakaiensis.
Int.J.Biol.Macromol., 243:125252-125252, 2023

PubMed Abstract: Ideonella sakaiensis is the bacterium that can survive by degrading polyethylene terephthalate (PET) plastic, and terephthalic acid (TPA) binding protein (IsTBP) is an essential periplasmic protein for uptake of TPA into the cytosol for complete degradation of PET. Here, we demonstrated that IsTBP has remarkably high specificity for TPA among 33 monophenolic compounds and two 1,6-dicarboxylic acids tested. Structural comparisons with 6-carboxylic acid binding protein (RpAdpC) and TBP from Comamonas sp. E6 (CsTphC) revealed the key structural features that contribute to high TPA specificity and affinity of IsTBP. We also elucidated the molecular mechanism underlying the conformational change upon TPA binding. In addition, we developed the IsTBP variant with enhanced TPA sensitivity, which can be expanded for the use of TBP as a biosensor for PET degradation.

PubMed: 37295700
DOI: 10.1016/j.ijbiomac.2023.125252

実験手法

X-RAY DIFFRACTION (1.02 Å)