8HJC

Solution structure of cysteine-rich peptide Bidentatide (Achyranthes bidentata peptide)

Summary for 8HJC

• Entry DOI: 10.2210/pdb8hjc/pdb
• Descriptor: Bidentatide (1 entity in total)
• Functional Keywords: cysteine rich peptides, plant protein
• Biological source: Achyranthes bidentata
• Total number of polymer chains: 1
• Total formula weight: 3425.99

Authors

Feng, Y.,He, M.,Zhang, X. (deposition date: 2022-11-23, release date: 2022-12-07, Last modification date: 2024-10-23)

Primary citation

He, M.,Feng, Y.,Wang, Y.,Cheng, M.,Zhang, X.,Zhang, L.
Discovery of a cysteine-rich peptide with glycation modification from Achyranthes bidentata Blume.
Fitoterapia, 163:105338-105338, 2022

PubMed Abstract: Cysteine-rich peptides (CRPs) are stable molecules that contain multiple disulphide bonds. Various CRPs are found in plants and animals, representing potential compounds for drug development with diverse activities. Modification of CRPs, such as glycation, has attracted increased attention due to its special structural and functional properties. Hence, this study explored a CRP isolated from the Chinese herb Achyranthes bidentata Blume, which contains a glycation modification. Herein, a reverse phase high-performance liquid chromatography system with mobile phases was used to extract and purify the peptide. The eluted peptide was detected using high resolution mass spectrometry and structurally identified using high resolution mass spectrometry and nuclear magnetic resonance. The effect of the peptide on the viability of N-methyl-D-aspartic acid (NMDA)-induced HT22 cells was determined using a cell assay. Here, a new cysteine-rich glycation peptide, termed glycation-bidentatide (Gly-BTP), with three pairs of disulphide bonds and a glycation modification at the N-terminus linked to cysteine, was discovered. Cell bioactivity assay results suggested that Gly-BTP might be a potential therapeutic and provide a neuroprotective effect in NMDA-induced HT22 murine hippocampal neuronal cells. The discovery of Gly-BTP will promote the understanding of the role of CRPs in neuroprotection.

PubMed: 36270560
DOI: 10.1016/j.fitote.2022.105338

Experimental method

SOLUTION NMR