8HIF

One asymmetric unit of Singapore grouper iridovirus capsid

This is a non-PDB format compatible entry.

Summary for 8HIF

• Entry DOI: 10.2210/pdb8hif/pdb
• EMDB information: 34227 34229 34230 34235 34236 34251 34815
• Descriptor: Major capsid protein, VP38, VP137, ... (7 entities in total)
• Functional Keywords: singapore grouper iridovirus, capsid proteins, viral protein
• Biological source: Singapore grouper iridovirus; More
• Total number of polymer chains: 144
• Total formula weight: 6703735.93

Authors

Zhao, Z.N.,Liu, C.C.,Zhu, D.J.,Qi, J.X.,Zhang, X.Z.,Gao, G.F. (deposition date: 2022-11-20, release date: 2023-04-19, Last modification date: 2024-10-23)

Primary citation

Zhao, Z.,Huang, Y.,Liu, C.,Zhu, D.,Gao, S.,Liu, S.,Peng, R.,Zhang, Y.,Huang, X.,Qi, J.,Wong, C.C.L.,Zhang, X.,Wang, P.,Qin, Q.,Gao, G.F.
Near-atomic architecture of Singapore grouper iridovirus and implications for giant virus assembly.
Nat Commun, 14:2050-2050, 2023

PubMed Abstract: Singapore grouper iridovirus (SGIV), one of the nucleocytoviricota viruses (NCVs), is a highly pathogenic iridovirid. SGIV infection results in massive economic losses to the aquaculture industry and significantly threatens global biodiversity. In recent years, high morbidity and mortality in aquatic animals have been caused by iridovirid infections worldwide. Effective control and prevention strategies are urgently needed. Here, we present a near-atomic architecture of the SGIV capsid and identify eight types of capsid proteins. The viral inner membrane-integrated anchor protein colocalizes with the endoplasmic reticulum (ER), supporting the hypothesis that the biogenesis of the inner membrane is associated with the ER. Additionally, immunofluorescence assays indicate minor capsid proteins (mCPs) could form various building blocks with major capsid proteins (MCPs) before the formation of a viral factory (VF). These results expand our understanding of the capsid assembly of NCVs and provide more targets for vaccine and drug design to fight iridovirid infections.

PubMed: 37041173
DOI: 10.1038/s41467-023-37681-9

Experimental method

ELECTRON MICROSCOPY (3.5 Å)