8HIA

Structure of transforming growth factor beta induced protein (TGFBIp) G623R fibril

8HIA の概要

• エントリーDOI: 10.2210/pdb8hia/pdb
• 関連するPDBエントリー: 8HGA
• EMDBエントリー: 34813
• 分子名称: Transforming growth factor-beta-induced protein ig-h3 (1 entity in total)
• 機能のキーワード: pathological fibrils, tgfbi related corneal dystrophy, protein fibril
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 20
• 化学式量合計: 50958.44

構造登録者

Low, J.Y.K.,Pervushin, K. (登録日: 2022-11-19, 公開日: 2023-07-26, 最終更新日: 2024-02-21)

主引用文献

Low, J.Y.K.,Shi, X.,Anandalakshmi, V.,Neo, D.,Peh, G.S.L.,Koh, S.K.,Zhou, L.,Abdul Rahim, M.K.,Boo, K.,Lee, J.,Mohanram, H.,Alag, R.,Mu, Y.,Mehta, J.S.,Pervushin, K.
Release of frustration drives corneal amyloid disaggregation by brain chaperone.
Commun Biol, 6:348-348, 2023

PubMed Abstract: TGFBI-related corneal dystrophy (CD) is characterized by the accumulation of insoluble protein deposits in the corneal tissues, eventually leading to progressive corneal opacity. Here we show that ATP-independent amyloid-β chaperone L-PGDS can effectively disaggregate corneal amyloids in surgically excised human cornea of TGFBI-CD patients and release trapped amyloid hallmark proteins. Since the mechanism of amyloid disassembly by ATP-independent chaperones is unknown, we reconstructed atomic models of the amyloids self-assembled from TGFBIp-derived peptides and their complex with L-PGDS using cryo-EM and NMR. We show that L-PGDS specifically recognizes structurally frustrated regions in the amyloids and releases those frustrations. The released free energy increases the chaperone's binding affinity to amyloids, resulting in local restructuring and breakage of amyloids to protofibrils. Our mechanistic model provides insights into the alternative source of energy utilized by ATP-independent disaggregases and highlights the possibility of using these chaperones as treatment strategies for different types of amyloid-related diseases.

PubMed: 36997596
DOI: 10.1038/s42003-023-04725-1

実験手法

ELECTRON MICROSCOPY (4.9 Å)