8HGW

Crystal structure of MehpH in complex with MBP

8HGW の概要

• エントリーDOI: 10.2210/pdb8hgw/pdb
• 分子名称: Monoalkyl phthalate hydrolase, 1-BUTANOL, PHTHALIC ACID (3 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Gordonia sp. P8219
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 126732.31

構造登録者

Zhang, Z.M.,Wang, Y.J.,Chen, Y.B. (登録日: 2022-11-15, 公開日: 2023-03-15, 最終更新日: 2024-10-30)

主引用文献

Chen, Y.,Wang, Y.,Xu, Y.,Sun, J.,Yang, L.,Feng, C.,Wang, J.,Zhou, Y.,Zhang, Z.M.,Wang, Y.
Molecular insights into the catalytic mechanism of plasticizer degradation by a monoalkyl phthalate hydrolase.
Commun Chem, 6:45-45, 2023

PubMed Abstract: Phthalate acid esters (PAEs), a group of xenobiotic compounds used extensively as plasticizers, have attracted increasing concern for adverse effects to human health and the environment. Microbial degradation relying on PAE hydrolases is a promising treatment. However, only a limited number of PAE hydrolases were characterized to date. Here we report the structures of MehpH, a monoalkyl phthalate (MBP) hydrolase that catalyzes the reaction of MBP to phthalic acid and the corresponding alcohol, in apo and ligand-bound form. The structures reveal a positively-charged catalytic center, complementary to the negatively-charged carboxyl group on MBP, and a penetrating tunnel that serves as exit of alcohol. The study provides a first glimpse into the enzyme-substrate binding model for PAE hydrolases, leading strong support to the development of better enzymes in the future.

PubMed: 36859434
DOI: 10.1038/s42004-023-00846-0

実験手法

X-RAY DIFFRACTION (2.80001173333 Å)