8HEH

Crystal structure of GCN5-related N-acetyltransferase 05790

Summary for 8HEH

• Entry DOI: 10.2210/pdb8heh/pdb
• Descriptor: GNAT family N-acetyltransferase, GLYCEROL, COENZYME A, ... (4 entities in total)
• Functional Keywords: gnat 05790, transferase
• Biological source: Serratia marcescens
• Total number of polymer chains: 2
• Total formula weight: 39025.28

Authors

Xu, M.X.,Ran, T.T.,Wang, W. (deposition date: 2022-11-08, release date: 2022-12-21, Last modification date: 2024-05-29)

Primary citation

Xu, M.,Liu, X.,Li, X.,Chen, L.,Li, S.,Sun, B.,Xu, D.,Ran, T.,Wang, W.
Crystal structure of prodigiosin binding protein PgbP, a GNAT family protein, in Serratia marcescens FS14.
Biochem.Biophys.Res.Commun., 640:73-79, 2022

PubMed Abstract: Acetylation is a conserved modification catalyzed by acetyltransferases that play prominent roles in a large number of biological processes. Members of the general control non-repressible 5 (GCN5)-N-acetyltransferase (GNAT) protein superfamily are widespread in all kingdoms of life and are characterized by highly conserved catalytic fold, and can acetylate a wide range of substrates. Although the structures and functions of numerous eukaryotic GNATs have been identified thus far, many GNATs in microorganisms remain structurally and functionally undescribed. Here, we determined the crystal structure of the putative GCN5-N-acetyltransferase PgbP in complex with CoA in Serratia marcescens FS14. Structural analysis revealed that the PgbP dimer has two cavities, each of which binds a CoA molecule via conserved motifs of the GNAT family. In addition, the biochemical studies showed that PgbP is a prodigiosin-binding protein with high thermal stability. To our knowledge, this is the first view of GNAT binding to secondary metabolites and it is also the first report of prodigiosin binding protein. Molecular docking and mutation experiments indicated that prodigiosin binds to the substrate binding site of PgbP. The structure-function analyses presented here broaden our understanding of the multifunctionality of GNAT family members and may infer the mechanism of the multiple biological activities of prodigiosin.

PubMed: 36502634
DOI: 10.1016/j.bbrc.2022.12.006

Experimental method

X-RAY DIFFRACTION (1.4 Å)