8HDR の概要
| エントリーDOI | 10.2210/pdb8hdr/pdb |
| EMDBエントリー | 34678 |
| 分子名称 | pam3 tube protein, Pam3 connector protein, Pam3 terminator protein, ... (5 entities in total) |
| 機能のキーワード | fiber, virus, viral protein |
| 由来する生物種 | uncultured cyanophage 詳細 |
| タンパク質・核酸の鎖数 | 54 |
| 化学式量合計 | 1122954.45 |
| 構造登録者 | |
| 主引用文献 | Yang, F.,Jiang, Y.L.,Zhang, J.T.,Zhu, J.,Du, K.,Yu, R.C.,Wei, Z.L.,Kong, W.W.,Cui, N.,Li, W.F.,Chen, Y.,Li, Q.,Zhou, C.Z. Fine structure and assembly pattern of a minimal myophage Pam3. Proc.Natl.Acad.Sci.USA, 120:e2213727120-e2213727120, 2023 Cited by PubMed Abstract: The myophage possesses a contractile tail that penetrates its host cell envelope. Except for investigations on the bacteriophage T4 with a rather complicated structure, the assembly pattern and tail contraction mechanism of myophage remain largely unknown. Here, we present the fine structure of a freshwater cyanophage Pam3, which has an icosahedral capsid of ~680 Å in diameter, connected via a three-section neck to an 840-Å-long contractile tail, ending with a three-module baseplate composed of only six protein components. This simplified baseplate consists of a central hub-spike surrounded by six wedge heterotriplexes, to which twelve tail fibers are covalently attached via disulfide bonds in alternating upward and downward configurations. In vitro reduction assays revealed a putative redox-dependent mechanism of baseplate assembly and tail sheath contraction. These findings establish a minimal myophage that might become a user-friendly chassis phage in synthetic biology. PubMed: 36656854DOI: 10.1073/pnas.2213727120 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (3.66 Å) |
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