8H8V

Room-temperature structure of lysozyme by pink-beam serial crystallography (100 ms, edge)

8H8V の概要

• エントリーDOI: 10.2210/pdb8h8v/pdb
• 分子名称: Lysozyme C, CHLORIDE ION, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: pink-beam, serial synchrotron crystallography, room-temperature, hydrolase
• 由来する生物種: Gallus gallus (chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14425.06

構造登録者

Kim, Y.,Nam, K.H. (登録日: 2022-10-24, 公開日: 2023-11-01, 最終更新日: 2026-03-04)

主引用文献

Kim, Y.,Nam, K.H.
Data of pink-beam serial synchrotron crystallography at the Pohang Light Source II.
Data Brief, 52:109811-109811, 2024

PubMed Abstract: Serial synchrotron crystallography (SSX) helps to determine the room-temperature structure of macromolecules with minimal radiation damage. Pink-beam X-ray provides more photon flux than a monochromatic beam, which can increase the diffraction intensity of crystal samples and reduce the issue of partial reflection measurement compared with a monochromatic beam. The demonstration of pink-beam SSX at the 1C beamline at the Pohang Light Source II (PLS-II) was previously reported. The Bragg peaks observed in SSX diffraction data using a pink-beam exhibited a slightly stretched shape, unlike that from a monochromatic beam. Therefore, it is necessary to develop an indexing algorithm that can efficiently process the Bragg peak generated by pink-beam SSX. Therefore, the collected pink-beam SSX diffraction data can be tentatively used to develop an indexing program for Bragg peaks generated using the pink-beam. In this study, detailed information on the diffraction data of pink-beam SSX at PLS-II was reported to access the raw data and process the information.

PubMed: 38161663
DOI: 10.1016/j.dib.2023.109811

実験手法

X-RAY DIFFRACTION (1.7 Å)