8H8M

Crystal structure of apo-E53F/E57F/E60F/E64F-rHLFr

8H8M の概要

• エントリーDOI: 10.2210/pdb8h8m/pdb
• 分子名称: Ferritin light chain, 1,2-ETHANEDIOL, CADMIUM ION, ... (6 entities in total)
• 機能のキーワード: ferritin, metal binding protein
• 由来する生物種: Equus caballus (horse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21071.53

構造登録者

Hishikawa, Y.,Noya, H.,Maity, B.,Abe, S.,Ueno, T. (登録日: 2022-10-23, 公開日: 2023-10-04)

主引用文献

Hishikawa, Y.,Noya, H.,Nagatoishi, S.,Yoshidome, T.,Maity, B.,Tsumoto, K.,Abe, S.,Ueno, T.
Elucidating Conformational Dynamics and Thermostability of Designed Aromatic Clusters by Using Protein Cages.
Chemistry, 29:e202300488-e202300488, 2023

PubMed Abstract: Multiple aromatic residues assemble to form higher ordered structures known as "aromatic clusters" in proteins and play essential roles in biological systems. However, the stabilization mechanism and dynamic behavior of aromatic clusters remain unclear. This study describes designed aromatic interactions confined within a protein cage to reveal how aromatic clusters affect protein stability. The crystal structures and calorimetric measurements indicate that the formation of inter-subunit phenylalanine clusters enhance the interhelix interactions and increase the melting temperature. Theoretical calculations suggest that this is caused by the transformation of the T-shaped geometry into π-π stacking at high temperatures, and the hydration entropic gain. Thus, the isolated nanoenvironment in a protein cage allows reconstruction and detailed analysis of multiple clustering residues for elucidating the mechanisms of various biomolecular interactions in nature which can be applied to design of bionanomaterials.

PubMed: 37070368
DOI: 10.1002/chem.202300488

実験手法

X-RAY DIFFRACTION (1.5 Å)