8H0Y

Structure of SARS-CoV-1 Spike Protein with Engineered x1 Disulfide (S370C and D967C), Locked-112 Conformation

8H0Y の概要

• エントリーDOI: 10.2210/pdb8h0y/pdb
• EMDBエントリー: 34418
• 分子名称: Spike glycoprotein, LINOLEIC ACID, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: protein engineering, spike protein, sars-cov-1, sars-cov, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 422802.92

構造登録者

Zhang, X.,Li, Z.,Liu, Y.,Wang, J.,Fu, L.,Wang, P.,He, J.,Xiong, X. (登録日: 2022-09-30, 公開日: 2022-11-09, 最終更新日: 2023-07-19)

主引用文献

Zhang, X.,Li, Z.,Zhang, Y.,Liu, Y.,Wang, J.,Liu, B.,Chen, Q.,Wang, Q.,Fu, L.,Wang, P.,Zhong, X.,Jin, L.,Yan, Q.,Chen, L.,He, J.,Zhao, J.,Xiong, X.
Disulfide stabilization reveals conserved dynamic features between SARS-CoV-1 and SARS-CoV-2 spikes.
Life Sci Alliance, 6:-, 2023

PubMed Abstract: SARS-CoV-2 spike protein (S) is structurally dynamic and has been observed by cryo-EM to adopt a variety of prefusion conformations that can be categorized as locked, closed, and open. S-trimers adopting locked conformations are tightly packed featuring structural elements incompatible with RBD in the "up" position. For SARS-CoV-2 S, it has been shown that the locked conformations are transient under neutral pH. Probably because of their transience, locked conformations remain largely uncharacterized for SARS-CoV-1 S. In this study, we introduced x1, x2, and x3 disulfides into SARS-CoV-1 S. Some of these disulfides have been shown to preserve rare locked conformations when introduced to SARS-CoV-2 S. Introduction of these disulfides allowed us to image a variety of locked and other rare conformations for SARS-CoV-1 S by cryo-EM. We identified bound cofactors and structural features that are associated with SARS-CoV-1 S locked conformations. We compare newly determined structures with other available spike structures of SARS-related CoVs to identify conserved features and discuss their possible functions.

PubMed: 37402591
DOI: 10.26508/lsa.202201796

実験手法

ELECTRON MICROSCOPY (2.85 Å)