8H0V

RNA polymerase II transcribing a chromatosome (type I)

8H0V の概要

• エントリーDOI: 10.2210/pdb8h0v/pdb
• EMDBエントリー: 34415
• 分子名称: DNA-directed RNA polymerase subunit, RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III, RNA polymerase II subunit B12.5, ... (22 entities in total)
• 機能のキーワード: chromatin, nucleosome, transcription
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 810891.01

構造登録者

Hirano, R.,Ehara, H.,Tomoya, K.,Takizawa, Y.,Sekine, S.,Kurumizaka, H. (登録日: 2022-09-30, 公開日: 2022-12-07, 最終更新日: 2024-07-03)

主引用文献

Hirano, R.,Ehara, H.,Kujirai, T.,Uejima, T.,Takizawa, Y.,Sekine, S.I.,Kurumizaka, H.
Structural basis of RNA polymerase II transcription on the chromatosome containing linker histone H1.
Nat Commun, 13:7287-7287, 2022

PubMed Abstract: In chromatin, linker histone H1 binds to nucleosomes, forming chromatosomes, and changes the transcription status. However, the mechanism by which RNA polymerase II (RNAPII) transcribes the DNA in the chromatosome has remained enigmatic. Here we report the cryo-electron microscopy (cryo-EM) structures of transcribing RNAPII-chromatosome complexes (forms I and II), in which RNAPII is paused at the entry linker DNA region of the chromatosome due to H1 binding. In the form I complex, the H1 bound to the nucleosome restricts the linker DNA orientation, and the exit linker DNA is captured by the RNAPII DNA binding cleft. In the form II complex, the RNAPII progresses a few bases ahead by releasing the exit linker DNA from the RNAPII cleft, and directly clashes with the H1 bound to the nucleosome. The transcription elongation factor Spt4/5 masks the RNAPII DNA binding region, and drastically reduces the H1-mediated RNAPII pausing.

PubMed: 36435862
DOI: 10.1038/s41467-022-35003-z

実験手法

ELECTRON MICROSCOPY (3.8 Å)