8H06

Cryo-EM structure of SARS-CoV-2 Omicron BA.4/5 RBD in complex with human ACE2 (local refinement)

8H06 の概要

• エントリーDOI: 10.2210/pdb8h06/pdb
• EMDBエントリー: 34409
• 分子名称: Processed angiotensin-converting enzyme 2, Spike protein S1, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: sars-cov-2, omicron ba.4/5, spike protein, viral protein, hydrolase-viral protein complex, hydrolase/viral protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 93211.77

構造登録者

Zhao, Z.N.,Xie, Y.F.,Qi, J.X.,Gao, G.F. (登録日: 2022-09-28, 公開日: 2023-07-19, 最終更新日: 2023-08-02)

主引用文献

Zhao, Z.,Xie, Y.,Bai, B.,Luo, C.,Zhou, J.,Li, W.,Meng, Y.,Li, L.,Li, D.,Li, X.,Li, X.,Wang, X.,Sun, J.,Xu, Z.,Sun, Y.,Zhang, W.,Fan, Z.,Zhao, X.,Wu, L.,Ma, J.,Li, O.Y.,Shang, G.,Chai, Y.,Liu, K.,Wang, P.,Gao, G.F.,Qi, J.
Structural basis for receptor binding and broader interspecies receptor recognition of currently circulating Omicron sub-variants.
Nat Commun, 14:4405-4405, 2023

PubMed Abstract: Multiple SARS-CoV-2 Omicron sub-variants, such as BA.2, BA.2.12.1, BA.4, and BA.5, emerge one after another. BA.5 has become the dominant strain worldwide. Additionally, BA.2.75 is significantly increasing in some countries. Exploring their receptor binding and interspecies transmission risk is urgently needed. Herein, we examine the binding capacities of human and other 28 animal ACE2 orthologs covering nine orders towards S proteins of these sub-variants. The binding affinities between hACE2 and these sub-variants remain in the range as that of previous variants of concerns (VOCs) or interests (VOIs). Notably, R493Q reverse mutation enhances the bindings towards ACE2s from humans and many animals closely related to human life, suggesting an increased risk of cross-species transmission. Structures of S/hACE2 or RBD/hACE2 complexes for these sub-variants and BA.2 S binding to ACE2 of mouse, rat or golden hamster are determined to reveal the molecular basis for receptor binding and broader interspecies recognition.

PubMed: 37479708
DOI: 10.1038/s41467-023-39942-z

実験手法

ELECTRON MICROSCOPY (2.66 Å)