8GXK

Pseudomonas jinjuensis N-acetyltransferase

8GXK の概要

• エントリーDOI: 10.2210/pdb8gxk/pdb
• 分子名称: Protein N-acetyltransferase, RimJ/RimL family, COENZYME A (3 entities in total)
• 機能のキーワード: complex with accoa, transferase, acetyltransferase
• 由来する生物種: Pseudomonas jinjuensis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 88460.54

構造登録者

Song, Y.J.,Bao, R. (登録日: 2022-09-20, 公開日: 2022-10-12, 最終更新日: 2023-11-08)

主引用文献

Song, Y.,Zhang, S.,Ye, Z.,Song, Y.,Chen, L.,Tong, A.,He, Y.,Bao, R.
The novel type II toxin-antitoxin PacTA modulates Pseudomonas aeruginosa iron homeostasis by obstructing the DNA-binding activity of Fur.
Nucleic Acids Res., 50:10586-10600, 2022

PubMed Abstract: Type II toxin-antitoxin (TA) systems are widely distributed in bacterial and archaeal genomes and are involved in diverse critical cellular functions such as defense against phages, biofilm formation, persistence, and virulence. GCN5-related N-acetyltransferase (GNAT) toxin, with an acetyltransferase activity-dependent mechanism of translation inhibition, represents a relatively new and expanding family of type II TA toxins. We here describe a group of GNAT-Xre TA modules widely distributed among Pseudomonas species. We investigated PacTA (one of its members encoded by PA3270/PA3269) from Pseudomonas aeruginosa and demonstrated that the PacT toxin positively regulates iron acquisition in P. aeruginosa. Notably, other than arresting translation through acetylating aminoacyl-tRNAs, PacT can directly bind to Fur, a key ferric uptake regulator, to attenuate its DNA-binding affinity and thus permit the expression of downstream iron-acquisition-related genes. We further showed that the expression of the pacTA locus is upregulated in response to iron starvation and the absence of PacT causes biofilm formation defect, thereby attenuating pathogenesis. Overall, these findings reveal a novel regulatory mechanism of GNAT toxin that controls iron-uptake-related genes and contributes to bacterial virulence.

PubMed: 36200834
DOI: 10.1093/nar/gkac867

実験手法

X-RAY DIFFRACTION (1.78 Å)