8GU6

Structure of the SbCas7-11-crRNA-NTR-Csx29 complex

8GU6 の概要

• エントリーDOI: 10.2210/pdb8gu6/pdb
• EMDBエントリー: 34270
• 分子名称: CHAT domain protein, RAMP superfamily protein, RNA (33-MER), ... (5 entities in total)
• 機能のキーワード: complex, rna-binding, rna binding protein
• 由来する生物種: Candidatus Scalindua brodae; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 296625.99

構造登録者

Yu, G.,Wang, X.,Deng, Z.,Zhang, H. (登録日: 2022-09-10, 公開日: 2023-01-18, 最終更新日: 2025-06-18)

主引用文献

Wang, X.,Yu, G.,Wen, Y.,An, Q.,Li, X.,Liao, F.,Lian, C.,Zhang, K.,Yin, H.,Wei, Y.,Deng, Z.,Zhang, H.
Target RNA-guided protease activity in type III-E CRISPR-Cas system.
Nucleic Acids Res., 50:12913-12923, 2022

PubMed Abstract: The type III-E CRISPR-Cas systems are newly identified adaptive immune systems in prokaryotes that use a single Cas7-11 protein to specifically cleave target RNA. Cas7-11 could associate with Csx29, a putative caspase-like protein encoded by the gene frequently found in the type III-E loci, suggesting a functional linkage between the RNase and protease activities in type III-E systems. Here, we demonstrated that target RNA recognition would stimulate the proteolytic activity of Csx29, and protein Csx30 is the endogenous substrate. More interestingly, while the cognate target RNA recognition would activate Csx29, non-cognate target RNA with the complementary 3' anti-tag sequence inhibits the enzymatic activity. Csx30 could bind to the sigma factor RpoE, which may initiate the stress response after proteolytic cleavage. Combined with biochemical and structural studies, we have elucidated the mechanisms underlying the target RNA-guided proteolytic activity of Csx29. Our work will guide further developments leveraging this simple RNA targeting system for RNA and protein-related applications.

PubMed: 36484100
DOI: 10.1093/nar/gkac1151

実験手法

ELECTRON MICROSCOPY (3.1 Å)