8GTC

Cryo-EM model of the marine siphophage vB_DshS-R4C baseplate-tail complex

This is a non-PDB format compatible entry.

Summary for 8GTC

• Entry DOI: 10.2210/pdb8gtc/pdb
• EMDB information: 34249
• Descriptor: Major tail protein, Distal tail protein, Megatron protein, ... (5 entities in total)
• Functional Keywords: marine bacteriophage, cryo-em, siphophage, baseplate, megatron protein, tail fibre protein, distal tail protein, hub protein, viral protein
• Biological source: Dinoroseobacter phage vB_DshS-R4C; More
• Total number of polymer chains: 27
• Total formula weight: 983646.39

Authors

Huang, Y.,Sun, H.,Wei, S.,Zheng, Q.,Li, S.,Zhang, R.,Xia, N. (deposition date: 2022-09-08, release date: 2023-07-12, Last modification date: 2024-06-19)

Primary citation

Huang, Y.,Sun, H.,Wei, S.,Cai, L.,Liu, L.,Jiang, Y.,Xin, J.,Chen, Z.,Que, Y.,Kong, Z.,Li, T.,Yu, H.,Zhang, J.,Gu, Y.,Zheng, Q.,Li, S.,Zhang, R.,Xia, N.
Structure and proposed DNA delivery mechanism of a marine roseophage.
Nat Commun, 14:3609-3609, 2023

PubMed Abstract: Tailed bacteriophages (order, Caudovirales) account for the majority of all phages. However, the long flexible tail of siphophages hinders comprehensive investigation of the mechanism of viral gene delivery. Here, we report the atomic capsid and in-situ structures of the tail machine of the marine siphophage, vB_DshS-R4C (R4C), which infects Roseobacter. The R4C virion, comprising 12 distinct structural protein components, has a unique five-fold vertex of the icosahedral capsid that allows genome delivery. The specific position and interaction pattern of the tail tube proteins determine the atypical long rigid tail of R4C, and further provide negative charge distribution within the tail tube. A ratchet mechanism assists in DNA transmission, which is initiated by an absorption device that structurally resembles the phage-like particle, RcGTA. Overall, these results provide in-depth knowledge into the intact structure and underlining DNA delivery mechanism for the ecologically important siphophages.

PubMed: 37330604
DOI: 10.1038/s41467-023-39220-y

Experimental method

ELECTRON MICROSCOPY (4.5 Å)