8GTB

Cryo-EM structure of the marine siphophage vB_DshS-R4C tail tube protein

これはPDB形式変換不可エントリーです。

8GTB の概要

• エントリーDOI: 10.2210/pdb8gtb/pdb
• EMDBエントリー: 34248
• 分子名称: Major tail protein (1 entity in total)
• 機能のキーワード: marine bacteriophage, cryo-em, siphophage, tail tube protein, viral protein
• 由来する生物種: Dinoroseobacter phage vB_DshS-R4C
• タンパク質・核酸の鎖数: 18
• 化学式量合計: 244346.69

構造登録者

Huang, Y.,Sun, H.,Wei, S.,Zheng, Q.,Li, S.,Zhang, R.,Xia, N. (登録日: 2022-09-08, 公開日: 2023-07-12, 最終更新日: 2024-06-19)

主引用文献

Huang, Y.,Sun, H.,Wei, S.,Cai, L.,Liu, L.,Jiang, Y.,Xin, J.,Chen, Z.,Que, Y.,Kong, Z.,Li, T.,Yu, H.,Zhang, J.,Gu, Y.,Zheng, Q.,Li, S.,Zhang, R.,Xia, N.
Structure and proposed DNA delivery mechanism of a marine roseophage.
Nat Commun, 14:3609-3609, 2023

PubMed Abstract: Tailed bacteriophages (order, Caudovirales) account for the majority of all phages. However, the long flexible tail of siphophages hinders comprehensive investigation of the mechanism of viral gene delivery. Here, we report the atomic capsid and in-situ structures of the tail machine of the marine siphophage, vB_DshS-R4C (R4C), which infects Roseobacter. The R4C virion, comprising 12 distinct structural protein components, has a unique five-fold vertex of the icosahedral capsid that allows genome delivery. The specific position and interaction pattern of the tail tube proteins determine the atypical long rigid tail of R4C, and further provide negative charge distribution within the tail tube. A ratchet mechanism assists in DNA transmission, which is initiated by an absorption device that structurally resembles the phage-like particle, RcGTA. Overall, these results provide in-depth knowledge into the intact structure and underlining DNA delivery mechanism for the ecologically important siphophages.

PubMed: 37330604
DOI: 10.1038/s41467-023-39220-y

実験手法

ELECTRON MICROSCOPY (3.43 Å)