8GSA

Solution structure of holo acyl carrier protein A from Enterococcus faecalis

8GSA の概要

• エントリーDOI: 10.2210/pdb8gsa/pdb
• 分子名称: Acyl carrier protein (1 entity in total)
• 機能のキーワード: acyl carrier protein, enterococcus faecalsi, biosynthetic protein
• 由来する生物種: Enterococcus faecalis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8619.44

構造登録者

Yeon, J.,Kim, Y. (登録日: 2022-09-05, 公開日: 2023-03-29, 最終更新日: 2024-05-08)

主引用文献

Yeon, J.,Oh, S.,Hwang, E.,Kim, E.,Kim, Y.
Structural study of acyl carrier protein of Enterococcus faecalis and its interaction with enzymes in de novo fatty acid synthesis.
Biochem.Biophys.Res.Commun., 637:232-239, 2022

PubMed Abstract: Enterococcus faecalis has recently shown signs of high antibiotic resistance. These bacteria can endure extremes of temperature and this may be due to the high thermostability of its proteins. E. faecalis has two acyl carrier proteins (ACPs), AcpA (EfAcpA), which is essential for de novo fatty acid synthesis (FAS), and EfAcpB, which plays an auxiliary role in the incorporation of exogenous fatty acids. Structural studies on EfAcpA and its interaction with FAS enzymes have not yet been reported. Here, we investigated the structures of EfAcpA using NMR spectroscopy, showing that EfAcpA consists of three α-helices with a long αα loop, while the other ACPs have four α-helices. CD experiments showed that the melting temperature of EfAcpA is 76.3 °C and the Ala mutation for Ile10 reduced it dramatically by 29.5 °C. Highly conserved Ile10 of EfAcpA mediates compact intramolecular packing and promotes high thermostability. A docking simulation of EfAcpA and β-ketoacyl-ACP synthase III (EfKAS III) showed that the αα loop of EfAcpA contributes to specific protein-protein interactions (PPI) with EfKAS III. Unconserved charged residues, Lys52 and Glu54, in the αα loop of EfAcpA formed specific electrostatic interactions with Asp 226 and Arg217 of EfKAS III, respectively. Binding interactions between EfAcpA and EfKASIII may provide insights for designing PPI inhibitors targeting FAS in E. faecalis to overcome its antibacterial resistance.

PubMed: 36410271
DOI: 10.1016/j.bbrc.2022.11.023

実験手法

SOLUTION NMR