8GRO

AtOSCA3.1 contracted state

8GRO の概要

• エントリーDOI: 10.2210/pdb8gro/pdb
• EMDBエントリー: 34210
• 分子名称: CSC1-like protein ERD4 (1 entity in total)
• 機能のキーワード: channel, membrane protein
• 由来する生物種: Arabidopsis thaliana (thale cress)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 164035.45

構造登録者

Zhang, M.F. (登録日: 2022-09-02, 公開日: 2023-06-14, 最終更新日: 2024-07-03)

主引用文献

Zhang, M.,Shan, Y.,Cox, C.D.,Pei, D.
A mechanical-coupling mechanism in OSCA/TMEM63 channel mechanosensitivity.
Nat Commun, 14:3943-3943, 2023

PubMed Abstract: Mechanosensitive (MS) ion channels are a ubiquitous type of molecular force sensor sensing forces from the surrounding bilayer. The profound structural diversity in these channels suggests that the molecular mechanisms of force sensing follow unique structural blueprints. Here we determine the structures of plant and mammalian OSCA/TMEM63 proteins, allowing us to identify essential elements for mechanotransduction and propose roles for putative bound lipids in OSCA/TMEM63 mechanosensation. Briefly, the central cavity created by the dimer interface couples each subunit and modulates dimeric OSCA/TMEM63 channel mechanosensitivity through the modulating lipids while the cytosolic side of the pore is gated by a plug lipid that prevents the ion permeation. Our results suggest that the gating mechanism of OSCA/TMEM63 channels may combine structural aspects of the 'lipid-gated' mechanism of MscS and TRAAK channels and the calcium-induced gating mechanism of the TMEM16 family, which may provide insights into the structural rearrangements of TMEM16/TMC superfamilies.

PubMed: 37402734
DOI: 10.1038/s41467-023-39688-8

実験手法

ELECTRON MICROSCOPY (3.5 Å)