8GJ3

E. coli clamp loader on primed template DNA

8GJ3 の概要

• エントリーDOI: 10.2210/pdb8gj3/pdb
• EMDBエントリー: 40084
• 分子名称: DNA polymerase III subunit delta, ZINC ION, DNA polymerase III subunit tau, ... (10 entities in total)
• 機能のキーワード: clamp loader, dna clamp, aaa+, atpase, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Escherichia coli K-12; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 319350.70

構造登録者

Oakley, A.J.,Xu, Z.-Q.,Dixon, N.E. (登録日: 2023-03-14, 公開日: 2024-03-27, 最終更新日: 2024-10-16)

主引用文献

Xu, Z.Q.,Jergic, S.,Lo, A.T.Y.,Pradhan, A.C.,Brown, S.H.J.,Bouwer, J.C.,Ghodke, H.,Lewis, P.J.,Tolun, G.,Oakley, A.J.,Dixon, N.E.
Structural characterisation of the complete cycle of sliding clamp loading in Escherichia coli.
Nat Commun, 15:8372-8372, 2024

PubMed Abstract: Ring-shaped DNA sliding clamps are essential for DNA replication and genome maintenance. Clamps need to be opened and chaperoned onto DNA by clamp loader complexes (CLCs). Detailed understanding of the mechanisms by which CLCs open and place clamps around DNA remains incomplete. Here, we present a series of six structures of the Escherichia coli CLC bound to an open or closed clamp prior to and after binding to a primer-template DNA, representing the most significant intermediates in the clamp loading process. We show that the ATP-bound CLC first binds to a clamp, then constricts to hold onto it. The CLC then expands to open the clamp with a gap large enough for double-stranded DNA to enter. Upon binding to DNA, the CLC constricts slightly, allowing clamp closing around DNA. These structures provide critical high-resolution snapshots of clamp loading by the E. coli CLC, revealing how the molecular machine works.

PubMed: 39333521
DOI: 10.1038/s41467-024-52623-9

実験手法

ELECTRON MICROSCOPY (2.8 Å)